活性蛋白在聚乳酸羟基乙酸薄膜表面的固定化及其生物学活性的分析-微生物学专业论文.docxVIP

cancer is stronger than esophageal cancer cells, the median lethal concentration of liver SMMC7721 IC50 value is 5.9×10-10 mol/L while the IC50 value of esophageal cancer EC109 is 1.984×10-6 mol/L, indicating that Ricin may be have selective cell-killing effects on different cancers. The possible anti-tumor mechanism of Ricin was studied. Giemsa staining and acridine orange (AO) fluorescence staining were used to observe the morphological changes of the liver cancer SMMC7721 and esophageal cancer EC109 treated with ricin, cell apoptosis and cell cycle distribution were detected by flow cytometry, whether DNA ladder is observed by Agarose Gel Electrophoresis if cells occur in apoptosis. Cell morphology and flow cytometry results of the test have indicated that at low concentrations Ricin inhibit the cell proliferation mainly by inducing cell apoptosis, but may lead to cell necrosis in higher concentrations or the effect time is too long. In this work, the active protein Bacitracin and Ricin were separately co-immobilized to the surface of PLGA membrane by using the carbodiimide (EDC-NHS) two-step chemistry grafting method. Through total reflection Fourier transform infrared (ATR-FTIR) technology we have made surface groups analysis of the PLGA membrane after active proteins were immobilized onto its surface. And then Staphylococcus aureus were used to detest antibacterial properties and liver cancer cell SMMC7721 were used to detect anti-tumor properties respectively after active protein immobilized to PLGA film. We have made a contrast between control PLGA membrane and co-immobilized active protein ones by MTT assay. Then assisting in acridine orange fluorescence staining pictures and photos of MTT we made a visual description of bacteria and cell adhesion on the surface of PLGA membrane. ATR-FTIR group’s characterization indicated that both of the active proteins were immobilized to the surface of PLGA film. MTT results showed that the active proteins have effecti