活血软坚方对兔颈脊髓慢性受压纤维化胶原纤维酸性蛋白表达的影响-中医骨伤科学专业论文.docxVIP

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活血软坚方对兔颈脊髓慢性受压纤维化胶原纤维酸性蛋白表达的影响-中医骨伤科学专业论文.docx

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The effects to rabbits GFAP expression of wich chronic cervical spinal cord compression fibrosis by Huo Xue Ruan Jian Fang ABSTRACT Objective: Observe Huo Xue Ruan Jian Fang spinal neurons changes in the microenvironment of the rabbit carotid nerve cells after chronic pressure, evaluation of the anti-fibrotic effects. Methods: New Zealand rabbits were taken around 2.5kg 120, charles random number table 90 randomly selected, using animal models introduced Cai Qinlin modeling, upon completion of the model were randomly divided into: Huo Xue Ruan Jian Fang group, Huo Xue Fang group, modeling control group 28; Another 30 for the Control group. The completion of the third day after the model of feeding drugs, Namely: Huo Xue Ruan Jian Fang、 Huo Xue Fang、Saline、Saline，time of five weeks.The first two days after discontinuation of the specimens. Randomly selected Huo Xue Ruan Jian Fang group、Huo Xue Fang group、Modeling in the control group、Control group Each 10. Thoracotomy after anesthesia, to the ascending aorta perfusion sacrificed and the whole spinal cord, into 2.5% glutaraldehyde phosphate buffer fixed, interception of damage to adjacent segments were sliced by HE staining, immunohistochemical staining.Experimental data obtained by image analysis system pathology images obtained four adjacent sections of cervical spinal cord injury in rabbits unit area neurons, area; systems analysis obtained by imagepro plus nearby section of the cervical spinal cord compression unit area NF200 and GFAP expression rate, and then analyzed statistically. Results:(1)The number of nerve cells per unit area, the area ( χ ±s) comparison: ①Huo Xue Ruan Jian Fang group: Quantity（7.760±1.568）Area （1576.473±269.840）, ②Huo Xue Fang group: Quantity（6.380 ±0.565） Area （ 1268.792±128.374 ） , ③Modeling in the control group Quantity （ 3.890±0.378 ） Area （ 986.454±48.611 ） , ④Control group Quantity （9.000±1.261）Area（1659.432±154.558）. Contrast the differences between the groups w