甲磺酸加替沙星对小鼠的免疫调节作用研究-微生物药学专业论文.docxVIP

中文摘要＼＼(1929年FIemi 中文摘要 ＼＼(1929年FIemi ng发现青霉素，随后F1()roy和Chai n将之用于l临 床算墩得惊人的治疗效果，就标志着抗生素时代的来临。现在抗生 素的研究不仅仅局限于对感染性疾病的治疗，而且已扩展到抗生素 对免疫系统的调节作用。寻找具有免疫调节作用的抗生素对免疫功 能受到j节制的肿瘤病人和免疫功能低下的老年病人的选择用药尤其 吖 重要。77 本文研究了甲磺酸加替沙星对小鼠非特异性免疫和特异性免疫 功能的调节作用，结果表明： 1．甲磺酸加替沙星(GFLX)有促进小鼠非特异性免疫功能的作 用。体外实验结果显示该药在0．5 MIC能促进小鼠腹腔巨噬细胞对 金黄色葡萄球菌的吞噬与杀菌作用(p0．01)；0．5 MIC预处理小鼠 腹腔巨噬细胞，也能促进其对金黄色葡萄球菌的吞噬与杀菌 (pO．01)。半体内实验结果显示小鼠尾静脉注射20mg／kg，24小时 后能促进体外腹腔巨噬细胞对金黄色葡萄球菌的吞噬与杀菌(pO．05 或p0，01)。体内实验结果显示小鼠预先口服10mg／l(g、20mgmg， 每日1次，连续3天，能抵抗金黄色葡萄球菌感染，对感染小鼠有 保护作用。 2．甲磺酸加替沙星能促进小鼠特异性免疫调节作用。腹腔注射 GFLX 10mg／kg和20mg／kg，连续5天，能显著提高小鼠特异性抗 SRBC抗体(溶血素)的产生(pO．01)，并呈剂量依赖关系。在0．05-40 u咖l范围内能协同ConA或LPS促进小鼠脾淋巴细胞转化。在 O．OI．10 pg／ml能抑制小鼠腹腔巨噬细胞产生，fNF“。小鼠腹腔注射 GFLXlomg／kg和20mg／kg“glb GFLXlomg／kg和20mg／kg“glb 9-re．著促进D1lH(p0．05或p0．01)，并 呈剖量依赖关系：对小鼠脾指数无明显影响。 ABSTRACTThe ABSTRACT The immunomodulatory effects of Gatifloxacin mesylate of mice This study investigated the immunomodulatory effects of gatifloxacin mesylate(GFLX)in·vitro．ex-vivo and in-vivo of mice．0．5 MIC of gatifloxacin mesylate enhanced mice macrophage phagocytosis and increased intracellular killing of S．aureus in vitro．After pre·exposure ofmacrophages to 0．5 MIC ofgatifloxacin mesylate，there was a significant increase in the intracellular killing of S．aureus compared with the controls；ex-vix O experiments show that iv administration of gatifloxacin mes)’late in mice lead to fin increase in phagocytic and intracellular bactericidal activities．In·vivo models of experimental infections with S． aureus showed that prophylactic administration of gatifloxacin mesy ate raised the survivaI of mice after challenge Gatifloxacin mesylate increased the production of anti—SRBC antibod)7 and the effects on mice splenocyte transformation in vitro stimulated with ConA and LPS wilhin O．05·40 u g／m1．Gafifloxacin mesylate inhibited the 3 production production ol、’INF“produced by normal mice peritoneal macrophages in vitro．Gatifloxacin mesylate also enhanced the delayed type hypersensitjVity in mice 4 氟喹