环氧化酶-2在Ⅲ期卵巢癌中的表达与化疗敏感性的关系-妇产科学专业论文.docxVIP

摘要目的检测环氧化酶一2(COX一2)在晚期卵巢癌中的表达，探讨 摘要 目的检测环氧化酶一2(COX一2)在晚期卵巢癌中的表达，探讨 其与卵巢癌化疗敏感性及其他临床病理因素的关系。 方法采用SP免疫组织化学的方法，对42例在我院手术、化疗 并有完整随访资料的III期卵巢癌和5例卵巢囊腺瘤及正常卵巢组织 COX一2的表达进行检测。用Fisher确切概率法分析COX一2与临床病 理因素之间的关系；生存率统计采用寿命表法和Kaplan—Meier法， 其差异性检验用log rank法；以患者对标准化疗的敏感性为因变量， COX一2的表达、年龄、病理类型、病理分级、化疗方案、化疗疗程为 自变量，进行Logistic回归分析。 结果42例卵巢癌组织中，COX一2表达阳性率为35．71％(15／42)， 5例卵巢良性囊腺瘤及正常卵巢组织表达均为阴性，具有显著性差异 (p0．01)。单因素分析表明COX一2的表达与病理分级、化疗疗程、 化疗敏感性显著相关(P0．05)，与患者年龄、病理类型无显著相关。 Kaplan—Meier生存曲线显示COX一2阴性者生存率较高(p0．05)。 Logistic回归分析表明COX一2的表达、病理分级和化疗疗程是卵巢 癌化疗敏感性的相关危险因素(PO．05)。 结论COX一2是影响化疗敏感性的独立因素，可能成为卵巢癌化 疗敏感性的新的标记物。 关键词环氧化酶一2，卵巢癌，化疗敏感性，免疫组织化学 主生剑堕皇堂焦鲨皇． 主生剑堕皇堂焦鲨皇． 墨苎塑墨 ABSTRACT objectives To investigate whether COX-2 expression in late stage ovarian patients relates to chemotherapy sensitivity and other factors Methods 42 paraffin-embedded tissue specimens were obtained from patients who received the first time cytoreductive surgery and chemotherapy in hospital and with complete follow-up detailes Tissue specimens were subjected to immunohistochemical analysis using polyclonal antibody to human COX-2．The correlation between COX一2 and several clinicopathological parameters was assessed by Fisher’S exact test．The probability of overall survival function of time was determined by Kaplan—Meier method and different survival Curves were compared by the log rank test．High risk factors of ovarian chemotherapy sensitivity were analysed by logistic regression．Generally， P values0．05 were considered significant．For the statistical evaluation the SPSS SOftware Version 1 2．0 was used Results Of 42 late stage ovarian patients，1 5(35．7 l％)were found COX--2 expression positive with diffuse COX-·2 immunostaining detected in the cytoplasm of the ovarian cells．COX一2 expression was significantly associated with tumor grade，chemotherapy and chemotherapy sensitivity，but not with his