马齿苋提取物对产ESBLs大肠杆菌体外抑菌作用及抑菌机理的研究-临床兽医学专业毕业论文.docxVIP

同提取物与12种常见抗菌药的联合抑菌效果，结果显示6种马齿苋提取物 同提取物与12种常见抗菌药的联合抑菌效果，结果显示6种马齿苋提取物 均有不同程度的联合抑菌效果，其中以马齿苋乙酸乙酯提取物和水提取物 的效果最好，乙醇提取物的效果次之。 探究了马齿苋水提取物与醇提取物对产ESBLs大肠杆菌的抑菌机理。 通过对大肠杆菌生长曲线的绘制，发现在马齿苋水提取物和醇提取物可以 抑制大肠杆菌的对数生长期，并呈浓度依赖性。通过透射电镜观察，药物 作用下的大肠杆菌细胞壁变薄，质壁分离，形成空腔。菌液碱性磷酸酶含 量测定和胞外可溶性蛋白含量测定发现马齿苋水提取和醇提取物可以影响 大肠杆菌细胞壁和细胞膜的结构，使细胞通透性增加。通过对胞内蛋白 SDS—PAGE电泳分析发现，马齿苋水提物和醇提取物可以抑制菌体蛋白的 合成。 关键词：马齿苋ESBLs 大肠杆菌抑菌作用 抑菌机理 万方数据 STUDY STUDY oN ANTIMICRoBIAL EFFECT AND MEC丑LANISM OF PDR丁【况4 C彳D乙ER4 C：Z王4三．AGAINST EXTENDED·SPECTRUM—p—LACTAMASE—PRODUCING ESCHERICHIA CoLI ABSTRACT Aiming at the high increasing rate of antibiotic resistance of bacteria from animals，The study was designed to observe the bacteriostatic action and bacteriostatic mechanism of Portulaca oleracea L． extracts against Drug-resistant E．coli．All above studies could supply for the treatment of colibacillosis in livestock and poultry and screening of new bacteriostatic agent from traditional Chinese medicine． The article used disk diffusion method to detect whether isolated E．coli strains producing ESBLs，and detected the ESBLs genotype by polymerase chain reaction，and tested the antibiotic sensitivity of 1 2 kinds of antimicrobial agents to the E．coli isolates by broth microdilution method．The results showed that isolates contain TEM and CTX—M genotype．And the isolated strain appeared diversely high resistance to 1 2 kinds of antimicrobial agents， especially for ceftriaxone，ceftofur,cefotaxime，amikacin，florfenicol and clindamycin，followed by quinolones． III 万方数据 In In addition，the bacteriostatic effect of 1 2 water．extracts of Chinese traditional medicines was detected．Portulaca oleracea L showed the best antibacterial effect to extended-spectrum 13-1actamase(ESBLs)and non—ESBLs producing Escherichia coli．The minimal inhibitory concentration respectively was 125 mg／mL、62．5 mg／mL．So Portulaca oleracea L was chosen for further study． The drug susceptibility testing of different polarity solvents extracts from Portulaca oleracea L showed the