慢病毒载体稳定转染人角质形成细胞克隆的基因表达谱和蛋白谱分析-外科学(烧伤)专业毕业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 1 缩略语表 2D two dimensional polyacrylamide gel electrophoresis 二维聚丙烯酰胺凝胶电泳 ACN acetonitrile 乙腈 CCL20 CC chemokine ligand 20 CC 亚族趋化因子配体 20 CHAPS 3-[(3-Cholamidopropyl)dimethylammonio] 3-[(3- 胆固醇氨丙基 ) 二甲基氨 基]-1-丙磺酸盐 cRNA complementary RNA 互补 RNA CT cycle threshhold 循环阈值 CV coefficient of variation 变异系数 Cy3 cyanine 3 青色素 3 DEPC diethyl pyrocarbonate 焦炭酸二乙酯 DTT dithiothreitol 二硫苏糖醇 EDTA ethylene diamine tetraacetic acid 乙二胺四乙酸 ERCC external RNA controls consortium 外部 RNA 质控簇 G418 geneticin 遗传霉素 GAPDH glyceraldehyde-phosphate dehydrogenase 磷酸甘油醛脱氢酶 GO gene ontology 基因本位学 HaCaT immortal human keratinocyte line 人永生化角质形成细胞系 HPV human papillomavirus 人乳头瘤病毒 iTRAQ isobaric tags for relative and absolute quantitation 同重同位素相对与绝对定量 LC-ESI-M S/MS liquid chromatography-electron spray ionization-mass spectra 液相色谱-电喷雾-四极杆飞行时 间串联质谱仪 lincRNA large intergenic noncoding RNA 长基因间非编码 RNA MAPK mitogen-activated protein kinase 丝裂原激活的蛋白激酶 OD optical density 光密度 PAGE polyacylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 PBS phosphate buffered saline 磷酸盐缓冲盐液 PMSF Phenylmethanesulfonyl fluoride 苯甲基磺酰氟 qPCR quantitative polymerase chain reaction 定量聚合酶链反应 RIN RNA integrity number RNA 完整指数 RPMI1640 Roswell Park Memorial Institute 1640 RPMI1640 培养基 RT-PCR reverse transcription-polymerase chain reaction 反转录酶-聚合酶链锁反应 SAM significant analysis of microarray 芯片差异显著性检测 SCX strong cation exchanger 强阳离子交换柱 SDS sodium dodecyl Sulfonate 十二烷基磺酸钠 shRNA short hairpin RNA 短发夹 RNA TEAB tetraethyl-ammonium bromide 溴化四乙铵 TNF tumor necrosis factor 肿瘤坏死因子 WB Western-blot 蛋白质印迹 The analyses of gene expression profiles and proteinograms of HaCaT cell clones with lentiviral-vector stable transfection Abstract In the curing of extensive burns, performing skin graft as soon as possible to minimize the area of wound plays a critical role for the survival of burn victims. However, skin autograft in severely burned patients is made difficult by the lack of autologous skin. So skin allograft a