抗血管内皮生长因子对糖尿病小鼠下肢缺血的治疗研究-药理学专业毕业论文.docxVIP

西南医科大学硕士学位论文LISA结果显示，糖尿病小鼠对照组缺血侧腓肠肌中VEGF．A的蛋白 西南医科大学硕士学位论文 LISA结果显示，糖尿病小鼠对照组缺血侧腓肠肌中VEGF．A的蛋白 水平明显高于同时间点的糖尿病小鼠贝伐单抗组VEGF．A的蛋白水 平。在第7天时，糖尿病小鼠对照组的VEGF蛋白水平最高。随着时 间的推移，贝伐单抗组的PDGF．BB蛋白含量逐渐升高，并且在第7、 14天时，糖尿病小鼠贝伐单抗组的PDGF．BB蛋白水平均高于同时间 点糖尿病小鼠对照组的PDGF．BB蛋白水平。4．体外细胞实验结果显 示：与其他组相比，贝伐单抗刺激人脐静脉内皮细胞分泌的PDGF．B B无明显差异。5．RT-PCR结果显示，糖尿病贝伐单抗组小鼠缺血侧 中PDGF．BB的表达量高于糖尿病对照组小鼠缺血侧PDGF．BB的表 达量。6．小动物活体成像结果显示：在第14天时，糖尿病小鼠贝伐 单抗组的血管渗漏程度明显低于糖尿病小鼠对照组。7．免疫荧光结果 显示：第7、14天时，各组缺血侧的微血管密度均大于第3天时缺血 侧的微血管密度。在第14天时，各组的微血管密度基本一致，但与 其他组相比，糖尿病小鼠贝伐单抗组周细胞覆盖程度最高。结论：1． 贝伐单抗可以促进糖尿病小鼠缺血下肢血流灌注的恢复。2．贝伐单抗 可上调PDGF．BB基因水平的表达。3．贝伐单抗减少血管渗漏、促进 血管成熟。4．贝伐单抗增加周细胞覆盖，促进新生血管成熟。 关键词：血管新生；血管内皮生长因子；糖尿病；下肢缺血 万方数据 西南医科大学硕士学位论文Anti—vascular 西南医科大学硕士学位论文 Anti—vascular endothelial growth factor treatment induces blood flow recovery through vascular remodeling in high—fat diet induced diabetic mice Abstract：Objective：To explore the mechanism of anti．vascular endothelial growth factor induces blood flow recovery in a mouse hind limb ischemia model．Methods：1．Feeding diabetic mice：high fat，sugar feeding about C5 7／BL6 mice about 1 4 weeks，tail vein blood glucose value was measured．2．Established model of hind limb ischemia：After anesthesia，Ligation of the left lower limb femoral artery,then cut the femoral artery between two ligations．After rewarming，laser Doppler scanner to scan blood flow in the lower limbs in mice，recording its perfusion recovery．Measurements were performed pre—and post．．artery ligation，additionally on postoperative days3，7，1 1 and 1 4．The left．to．right ratio(occluded—to—nonoccluded leg)was calculated for each animal．Mice were divided into four groups：control，bevacizumab in diabetic mice． control，and bevacizumab in C5 7／BL6 mice，Each group of 1 8 mice． Bevacizumab was intraperitoneally administered on the days of 1，3,7and 1 1·Saline as contr01．In some cases，by using small animal in vivo imaging system，vascular leakage fluorescent dye was used to monitor vascular leakage in mice．3．Gastrocnemius muscles were harvested for