黄芩苷在黄芩植株体内积累规律的研究-中药学专业毕业论文.docx

提要为了解黄芩植株体内黄芩苷的合成积累规律及其影响因素，达到控制或提高栽培 提要 为了解黄芩植株体内黄芩苷的合成积累规律及其影响因素，达到控制或提高栽培 药材质量的目的，我们采用种子发芽与盆栽方式，利用高效毛细管电泳、高效液相色 谱等手段，研究了黄芩苷在黄芩植株各器官中的分布及生长时间、温度、光照、营养 元素等因素对黄芩苷含量的影响。结果表明：黄芩种子不含黄芩苷，植株其它器官中 均含有，但含量多少依次为：根花叶茎，主根侧根支根，韧皮部木质部；种子 萌发至子叶展开时，黄芩苷才开始产生，随着幼苗不断长大含量逐步提高，一年生植 株的生长时间越长，根中黄芩苷的含量越高；光照具有促进黄芩苷合成的作用，光照 条件下生长幼苗中的黄芩苷含量显著高于黑暗条件下生长者，光照越强，植株根部的 黄芩苷含量越高：温度对黄芩苷的合成也有影响，以25。C最适。补充营养元素能促 进黄芩苷合成，在种子发芽时以钾肥和氮磷复合肥的促进作用最大，对砂培植株来讲t 铜有抑制作用，而磷、钾等元素有促进作用；在植株营养状况较差的情况下，补充蔗 糖与葡萄糖能够提高黄芩苷含量，正常植株补充蔗糖与葡萄糖时，黄芩苷的含量有所 降低：黄芩苷含量与可溶性蛋白质含量之间具有一定的相关性，在种予萌发幼苗生长 期间为明显的负相关性，在施肥补充营养元素后为明显的正相关性；各器官均有过氧 化物酶存在，但POD酶谱中的带1，只存在于根中，在茎、叶及花中存在较少或不存 在，既使在根中也会因生长时间不同而存在谱带宽度的差异；不同产地种子的蛋白电 泳图谱有明显差异，证明其遗传性发生了变化。 关键词黄芩：黄芩苷：外界因素；POD酶：蛋白质 Studies Studies on Baicalin Accumulating Law in the Plant of Scutellaria baicalensis Georgi Speciality：Chinese Medicine Author： Zhang Chunfeng Tutor： Zhang Yongqing Abstract To control and improve the quantity of the medicinal material by understanding baicalin accumulating law in the Scutellaria baicalensis georgi and external factors on accumulating baicalin，we studied haicalin distributing law in individual organs and the external factors to the content of baicalin such as temperature，light，fertilizer,and so fjml． The content of baicalin during germinating of seeds was monitored by HPLC and HPCE。 Individual tissues and segments of particular organs differ in their ability to accumulate secondary productS．Therefore，particular organs，individual parts and different level radix were our studying objects．Moreover,PAGE was used to indicate the changes of POD in the different growth phases and individual organs of the plant．Histochemistry method was also used to determine baicalin content in different parts． Studies have shown that the baicalin is in every organ rather than in the seeds；the content of baicalin in radix is higher than that of flowers，then in leaves and then in stems； That in taproot is highest，in turn is sideroot，and fibreroot；nle content in phloem is higher than that of