丙泊酚对大鼠急性脑损伤S100β蛋白、脑组织总钙、含水量及组织形态学的影响.doc

宁夏医科大学硕士研究生学位论文 英文摘要 Effect of propofol on S100β P rotein content, total c alc ium content, water content, and the brain tissue mo rpho lo g y of acute bra in injury in rats ABSTRSCT Obje ctive : Observation of propofol on acute brain injur y in rats according to serum S100 βProtein, brain tissue total calcium content, and water content, and the change of brain tissue morpholo gy. Explore propofol effect on acute brain injur y in rats. Methods: 84 healthy ma le SD rats are ra ndo mly d ivid ed into six gro up s with 14 rats each(n=14), sham gro up( gro up S), brain injury gro up( gro up I), fat e mulsio n gro up( gro up F), Low-dose propo fo l gro up( gro up L), M idd le-dose propofol gro up( gro up M) and High-dose propo fo l gro up( gro up H). By imp ro ved Fee ne y using freefall brain injury unit, established brain injury model in rats: SD rats are used 10% chloral hydrate to 350mg/kg are a ne sthe tized by intraperitoneal injection. Wait until d is appear of righting re fle x, eyelash reflex are pro mpted a nesthes ia success. The rats are fixed sup ine positio n on the operating tab le, endo trac hea l intubation, connect small animal ve ntila tio n, tidal vo lume 2-3ml/100g, respiratory rate 50-60 beats/min, inspiratory to exp ira tory ra tio 1 :2. Separating the le ft fe mora l ve in and catheter for intra ve no us drugs. Separating the right common carotid artery and catheter for b lood sampling. After arterial and ve no us are inserted, rats are flxed for prone pos itio n. With po vido ne- iod ine d isinfectio n of the skin. Aseptic opera tio n. Med ian inc is io n. Perio stea l stripp ing. Exposed to the right parietal bo ne. With a de nta l drill a fter the coronal suture 1.5mm, midline 2.5mm drilled a 5mm d ia meter bo ne window, keeping intact dura. Gasket is placed on the bo ne window, 30cm height with 20g weight free fall along the IV 万方数据 宁夏医科大学硕士研究生学位论文 英文摘要 casing, resulting in right parietal brain da ma ge, strik ing force 600 g·c m. After being