黄芩苷对实验性脑出血大鼠脑保护作用.doc

Abstract Abstract Intracerebral hemorrhage (ICH) is a serious disease that has high mortality， disability and fatality ratio without effective treatment. So it is still a hot point to research the treatments of ICH. Brain edema is one of the important factors of its high mortality. Increased the production of free radicals is reported to have close relationship with brain edema, and eventurally causes the death of neurons. Nervous excitotoxicity induced by EAAs could cause acute or chronic cell damage and cell death.The disequilibrium between EAAs and IAAs could cause brain histologic lesion. Inflammatory reaction was also discovered in ICH, cytokine as an important inflammatory factor.plays a important role in pathogenesis of ICH. Accordingly，to keep the kinetic equilibrium between free radicals, EAAs\ IAAs, and to inhibite the overexpression of cytokine is a domain which we are going to open up to treat pathobolism lesion of ICH. Rencently it has considerable curative effect to treat cerebrovascular disease with baicalin.It’s reported that baicalin has much biological function, such as protecting endotheliocyte,to getting rid of free radicals, decreasing cerebral ischemia-induced neuron injury, and so on. However, study on protective mechanism of baicalin on ICH was seldom reported. Rat model of intracerebral hemorrhage was induced by collagenase method. The rats were randomly divided into sham operation control group,model group, high， middle and low dose drug group.After treatment,to observe protection of baicalin on ICH, the brain water content and the level of SOD，MDA,NO and NOS were measured，and the morphology was also observed. At the same time, the level of Glu and GABA and its metabolic enzyme, GAD and GABA-T, were determined, and the expression of cytokine, TNF-α and IL-1β, were detected. The results were as following: III The Protection of Baicalin on Brain following Experimental Intracerebral Hemorrhage in Rats The brain water content and the level of