棉酚衍生物的合成及其生物活性研究-应用化学专业毕业论文.docx

.3. .3. 闸， 验结果表明，醋酸棉酣及其棉酣衍生物 1. 日与悦-DNA 形成了 1: 1 的复合物，且与 ct-DNA 的相互作用是通过嵌入作用方式嵌入 DNA 的政螺 旋之间，其大小l顺序是:棉酣衍生物 n 棉酣衍生物 1棉酣，棉酣衍生物 IU ，IV 与 ct-DNA 的相互作用较藉。 (4) 棉酣衍生物日与金属离子形成的配合物水解质粒 DNA 的研究 通过琼脂糖;烧胶电流的研究发现:棉酣衍生物 H 及真单独的铜离子和 解离子本身并没有切割 DNA 的活性，在铜商子或桦圈子的存在下，棉酣衍 生物口表现出了对 DNA 的切割活性。 关键词 E 棉酌棉酣衍生物细胞增殖括性结合常数凝胶电泳 . .4 SYNTHESIS THE GOSSYPOL DERIVATIVES AND STUDY ON THE BIOLOGICAL ACTIVITY ABSTRACT The problem about the process of ex阳.ctíng gossypol from the cotton seedwωstudied ín 也ispap由民也e gossypol derivatives ( their struc仙rewere studied by 盯阳血， IHNh也 as well as MS m础。ds) were syt由esized ，and then the biological activity such as the cell inhibition ，the ínteraction with 世le ct-DNA and hydrolysís ofthe plasmid DNA by metal complexes with the gossypol derivative 11 were discussed .四le experiment results were followíng: (1) Study on 也e production of gossypol from co扰。nseed The technology of the aniline-acetone was choosed ín 也is pap吼伽is isω say，the 仕ee gossypol was extracted 货。m the cotton seed with organíc reagent ， next reacted with the anilíne，then was converted ω 伽e acetic acid gossypol by the sulphuric acid . During the gossypol recrystalledωthe pure ? we used the aether and aceωne íntersection and antí- oxídation treatment.A11 these methods succeed ωsettlíng the problem that gossypol is oxidized easily .币le purity of 伽e acetic acid gossypol reached 10 也e expectant require. 。)Study on the cells ínhibition ofthe gossypol acetic acíd and its derivatives .5. .5. Gossypol acetic acid and its derivatives were foundωbe ableω 汹汹bit the swvíval of the K562 cell by 1\盯T assay，the inhibitíon rate of which are greater 伽础性le ci如Platinum( II)-D阳nmine Dichloride. The ICso of the gossypol derivative II is 5 times of gossypol acetic acid， 12 伽nes of gossypol derivative 1， 6 times of gossypol m and IY. Study on the interaction of gossypol derivatives with ct-DNA 币le interaction between derivatives of gossypol and ct-DNA were studied by UV-