抗精子抗体对精子顶体反应（形态学）的影响-泌尿外科专业毕业论文.docxVIP

抗精子抗体对精子顶体反应(形态学)的影响捅矍 抗精子抗体对精子顶体反应(形态学)的影响 捅矍 目的探讨精浆抗精子抗体对人精予顶体反应的影响。 方法利用ELISA法筛选出精浆中IgG类抗精子抗体阳性免疫性不育 患者，其精液离心后取其精浆同正常人精子孵育，同时设立正常精浆及精 子培养液与正常人精子孵育作为对照组，以孕酮诱发精予发生顶体反应； 利用特异性荧光标记物．络合异硫氰酸荧光素的豌豆凝集素(FITC—PSA)标 记精子顶体，该物质在488nm波长的激发光照射下，在510～550nm处发绿 色荧光，通过流式细胞仪检测荧光强度，间接反映各组标本顶体完整性， 评价抗精子抗体对项体反应的影响。 结果 以IgG类抗精子抗体阳性精浆孵育的精子，其孕酮诱发的顶体 反应发生率明显低于正常精浆及精子培养液组(P0．001)，正常精浆组及 精子培养液组间无显著性差异(P0．05)；lgG类抗精子抗体阳性精浆组、 正常精浆组、精子培养液组自发顶体反应的发生率无显著性差异(PO．05)。 结论免疫性不育患者精浆中的IgG类抗精子抗体可以明显抑制孕酮 诱发的顶体反应的发生。 关键词抗精子抗体，精子，顶体反应，免疫性不育 The The influence of antisperm antibody on the acrosome reaction(morphology) Abstract objective To explore the influence of IgG-anfisperm antibody in seminal plasma on the sperm acrosome reaction(AR)． Methods ELISA tests were performed in our study to select the IgG—antisperm antibody positive semens．The semens then were centrifuged and the seminal plasma were isolated，30 such samples were collected．Spermatozoa from ten healthy donors were incubated with those samples；they were compared to a control group of 10 samples without antisperm antibodies and 10 samples with buffer simultaneously．The spontaneous acrosome reaction(SAR)and the induced acrosome reaction OAR)by progesterone were determined by mearis of a flow c”ometer．The FITC—PSA was used to stain the sperm acrosome，which can radiate green fluorescence at 5 10-550nm wavelength as excited by 488nm wavelength laser．The value of FITC fluorescence was accumulated and calculated by a special computer as the stained sample passed the flow cytometer,the results were output as numeric value． Results The spermatozoa treated with IgG·antispema antibody positive seminal plasma showed significantly lower IAR responses than the two control groups(po．001)，but there was UO significant difference between control groups in IAR(p0．05)；and there was no significant difference between the three groups in SAR(p0．05)． Conclusion IgG-antisperm antibody from immunologic infertility patients is able to inhibit progesterone induced acrosome