慢病毒介导hHCN1和hHCN4基因不同比例体外转染骨髓间充质干细胞的表达及意义心血管内科学专业毕业论文.docxVIP

慢病毒介导 慢病毒介导 hHCN1 和 hHCN4 基因不同比例体外转染骨髓间充质干细胞的表达及意义 英文摘要 III III IV IV Expression and signifigance of bone marrow mesenchymal stem cells transfected with different proportions of hHCN1 and hHCN4 by lentivirus Abstract Background:Sick sinus syndrome is a common clinical disease,but the mechanism is yet to be defined. In the present medical condition, we mainly use the permanent electronic pacemaker. But with the advantages come with the disadvantages, such as limited battery life, the need for permanent implanation of electrodes into the heart and lack of response to nerve-humoral factors regulation. Lately, considering the physical function of heart and adaptability of human body, we bring up the concept of the biological pacemaker. The related current, If, encoded by the hyperpolarization-activated cyclic-nucleotide gated, plays an important role in the process of spontaneous diastolic depolarization of sinoatrial cells. Therefore, we put forward bone marrow mesenchymal stem cells(MSCs) transfected with HCN as cell platform to construct a “biological pacemaker” which provides experimental basis for the alternative to electronic pacemaker for clinical treatment in the future. Objective: We adopt the lentiv-GFP, carrying green fluorescent, as a vector to study the characteristics of the pacemaker current in the MSCs co-transfected with different proportions of hHCN1 and hHCN4. Meanwhile, we do some chemical tests on the cells transfected positive aimed at the establishment of cell–genetically modified organism pacing therapy experimental basis in vitro. Methods： Extraction, culture and purification of SD rat MSCs. Lentiv-hHCN1 and Lentiv-hHCN4 transfect and co-transfect the MSCs in different proportions. Lentiv-hHCN1 and Lentiv-hHCN4 in different proportions transfect the 3rd generation of MCS, at the same time, we set hHCN1 and hHCN4 individually transfecting MSCs and 英文摘要 慢病毒介导 hHCN1 和 hHCN4 基因不同比例体外转染骨髓间充质干细胞的表达及意义 lentiv