黄芩素对脑缺血小鼠运动功能恢复及轴突 生长、突触重塑相关因子的影响-神经病学专业毕业论文.docxVIP

万方数据 万方数据 目 录 中文摘要 ·············································································1 英文摘要 ·············································································3 英文缩写 ·············································································5 研究论文 黄芩素对脑缺血小鼠运动功能恢复及轴突生长、突触重塑 相关因子的影响 前言 ·············································································6 材料与方法 ····································································7 结果 ·············································································11 附图 ·············································································12 讨论 ·············································································17 结论 ·············································································19 参考文献 ·······································································20 综述 脑卒中后神经功能修复的研究进展 ·····································23 致谢 ···················································································36 个人简历 ·············································································37 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 黄芩素对脑缺血小鼠运动功能恢复及轴突 生长、突触重塑相关因子的影响 摘 要 目的：脑血管病是严重危害人类健康的重大疾病，脑卒中会导致严重 的神经功能缺损和身体残疾，由于大脑对受损神经功能的修复作用有限， 有关轴突再生和突触重塑与神经功能恢复的关系研究备受重视。黄芩素 （baicalein）是从黄芩等中药中提取的天然活性物质，具有抗炎，抗氧化， 抗凋亡等作用，已经被应用于多种疾病的治疗，但有关黄芩素在卒中后神 经功能恢复过程中所起的作用尚不明了。轴突膜蛋白（GAP43）， 脑源性 神经营养因子（BDNF）以及突触相关蛋白（PSD95 和 SYP）已被证实是 与轴突再生和突触重塑相关的因子，同样也与脑卒中后运动功能恢复密切 相关。本实验旨在观察黄芩素长期干预治疗后对实验性脑缺血小鼠运动功 能及轴突再生、突触重塑相关的因子的影响，探索黄芩素可能的作用机制， 为脑梗死的治疗提供新的靶点。 方法：雄性 C57BL/6 小鼠，烧灼法制备小鼠局灶皮层梗死模型。将 小鼠随机分为 4 组：假手术组（Sham）；梗死组（MCAO），黄芩素小 剂量组（Baic-L, 30 mg/kg）；黄芩素大剂量组（Baic-H, 100 mg/kg）。用 药组于术后给予黄芩素灌胃，一日一次，连续 2 周，各组小鼠于术后第 1、 3、7、14 天进行 Rotarod 行为学评分；假手术组（Sham）、梗死组（MCAO） 及黄芩素大剂量组（Baic-H）于术后第 7、14 天测定 GAP-43、BDNF、 PSD95、SYP 蛋白的表达。 结果： 1 黄芩素可明显改善实验性脑缺血小鼠运动功能。实验小鼠术后第 1 天及第 3 天，三个组处于平衡基线水平，三组之间无统计学差异（P 0.05）； 实验小鼠于术后第 7 天，Baic-H 组神经功能明显改善，与 MCAO 组和 Baic-L 组相比均有统计学差异（P 0.05），Baic-L 组与 MCAO 组比较无 统计学差异（P 0.05）；实验