链霉菌la5菌株摇瓶发酵条件优化与抗生素分离纯化-植物病理学专业论文.docxVIP

AbstractThe Abstract The Sf即幻删c嚣sp．LA5 s仃ain waLs isolated from the capsicum pl舡lt in H血an Pro也ce，C11ina，and s仃ongly inllibitor)r t0 P砂f印．}l砌Dm c印s捌，n‘s口一“m恻印口棚m f．sp．cH6明sB CDf比c幻frfc^“m m淞口e， etc．LA5’s f色衄en嘶on liquid of carrot gaVe good con廿01 e骶cts against capsicum blight’baIlana PaIl锄a dise2Lse aIld banana aIltll】阻cnose．The a11吐fungal actiVe component is the aIl曲iotics produced by LA5 s仃a_iIl， but itS producdon is low in LA5 f色men僦on Hquid of caIrot．h order to understand the aIl曲io丘c LA5 be仕er’we opti血zed t11e medium alld process paraJIleter in shaking flaLsk for LA5 fe锄en伽on，a11d pllrified tlle aIltifungal actiVe component丘om LA5 ferInentation Hquid． A total of 11 f如tors were tested meir funcdon of ilnproViIlg aIl曲io廿cs yield of LA5 by siIl91e—fj虻tor test IIlemods．The results showed mat glucose is tlle best cafbon fbr me andbio廿cs producd、，it)，of LA5，omer sucrose，maltose，conl nour；beaIl flour is t11e best Ili廿ogen sources，otllers peptone， yeast flouryeaSt exⅡ．act，beef ex缸act；divalent ions Ca2+，Mg“stimulated LA5 aIl曲iodc bios)，Ilthesis． Hereby’an un渤眦design test waS applied to叩tiIllize aboVe 4 factors．The fouow regression model equation aIld opdIIlized medium were obtailled according to regression analysis of me data丘Dm uIlifonn design exp e】riment：Y=一5．7512+0．5515X1+O．7811X2+26．6954X3+26．8706X4·0．0066X1‘-0．0115X／ 一24．0747X32—22．5914)(42—0．01302XlX2—0．3516X2X4—21．4559X3)【4．ne optiIIlum culture medium includes beall nollr 28．00 g／L，glucose 13．78 g／L，CaC03 0．42 g／L，MgS04 0．28 g／L The model waLs vaJidated eXp甜Inentally by tlle Value of 16．10111In，a11d tlle deviation was 4．62％coInpared丽m theoretical value．nle results also indicated that the op血num process paraIl：leter iIl sh挝ng naSk fer玎∞n伽on consist of initial pH of 6．8，28℃of culture，5％of iIlocul撕on amount，Inedium loadage 100I州300nll，200 r，IIlin of rotation．aIld 120h of f；巳nnentation血ne． rrhe purification procedure of血e‰oluble aIltibiotic LA5 waS elucidatedby research fermen雠on 1iquid pre臼e锄【nem，mta叮eVaporator con