两个抗玉米矮花叶病基因的精细定位和作用方式的研究-作物遗传育种专业论文.docxVIP

分析，对两个抗病基因的作用方式进行了研究，两年的研究结 分析，对两个抗病基因的作用方式进行了研究，两年的研究结 果表明：第六染色体上的抗病基因负责早期的抗病性，而第三 染色体上的抗病基因在后期起作用；两个抗病基因同时存在， 表现出全生育期抗病。 关键词玉米矮花叶病；显性互补；抗病基因；精细定位；基因作用 方式 Fine Fine mapping and analysis of mode of gene action of two resistance genes to maize dwarf mosaie virus Supervisor：Prof．Wu Jianyu Master Candidate：DU Yanxiu ABSTRACT Maize dwarfmosaic is ofthe devastating and widespread viral diseases caused by maize dwarf mosaic virus(MDMV)in the world．For ecological and economical reasons，the cultivation of resistant maize varieties is the most efficient method control virus disease．Two resistance genes were primarily mapped chromosome 3 and 6 in Siyi．In this study,fine mapping of two resistance genes WaS done with the aid of SSR markers and the resistance controlled by the two dominant complementary genes was testified．And the action mode of tWO resistance genes was studied．The results were f0110Ws： 1．Two hundred and ninety six F2 plants from the of Siyi×M017 was used map the two genes by SSR makers，which well distributed the maize genome， mostly distributing chromosome 3 and chromosome 6．Four SSR markers were screened from total of 148 SSR makers．The genetic distances between markers and genes were calculated，which showed markers pbi053 and umcl 527 flanking the resistance gene chromosome 3 were 1 cM respectively,markers phi075 and bnl91600 flanking the resistance gene chromosome 6 were 4 cM and tcM respectively． 2．The resistance conditioned by the two dominant complementary genes was confirmed by Mendel genetic analysis幻6 generations from the of Siyi×M01 7 and F3 progenies．The result was also testified by microsatellite analysis of F2 popuhtion． 3．Microsatellite analysis of F2 population by primers phi053 chromosome 3 and bnlgl600 chromosome 6，linking tightly with the two resistance genes respectively, was was used identify the action mode of the two resistance genes based the observation of resistance different stages．It revealed that the resistance gene chromosome 6