两亲壳聚糖水凝胶的合成及其在蛋白类药物释放体系中的应用-有机化学专业论文.docx

Synthesis Synthesis and application in protein drug delivery systems of amphiphilic chitosan hydrogels YANG Hua(Organic Chemistry) Directed by DENG Xian—mo Abstract With development of protein drug，biodegradable materials with amphiphilic structure have been investigated by scientists because these materials could remarkably enhance drug stability and biocompatibility．Biodegradable materials based on chflosan have attracted much attention because of the application of ecological situation．In this papeL amphiphilic chitosan hydrogels were modified into materials for protein drug delivery． Firstly,amphiphilic N—au(yl—lactosylated-chitosan were obtained by attaching lactose and alky!groups to the amino group of chitosan．When they were prepared to hydtogels，the hydrogels possessed adjustable amphiphilicity by swelling measure。 Hydrogels including N-alkyl-laetosylated-chitosan and bullfrog skin collagen were also prepared．The sweRing behaviours of the hydrogels were similar to N-alkyl—lactosylated-chitosan hydrogels． Secondly,N—alkyl-lactosylated-O，N-earboxymethylchitosan were synthesized by attaching lactose and al】bl groups to amino group of 0，N-carboxymethylchitosan． Hydrogels based on N—alkylqaetosylated-O，N-carboxymethylchitosan had pH-sensitivity With contrary to N．alkyl—lactosylated-chitosan,the pH-sensitive hydrogels had much larger swelling ratio in pH7．4 and pH9 buffers than that in pHl buffer． Lastly,Different amphiphilic hydrogels were synthesized bY chitosan and poly (1actic acid)(PLA)in various ways．(1)PLA was reacted with acryloyl chloride to obtain acrylated PLA，the acrylated PLA and chitosan was led to the formation interpenetrating network(IPN)hydrogels．(2)The lactic acid as its own catalyst for reaction reaction onto chitosan,the grafted chitosan was copolymerized with acrylated PLA tO form gel by using cross-linker．(3)The lactic acid was grafted onto hydroxyl groups of chitosan，physical cross—linking ofthe product could be introduced