磷脂酶D参与冬小麦对干旱胁迫的响应-中国农业气象.PDF

中国农业气象（Chinese Journal of Agrometeorology ） 2019 年 doi:10.3969/j.issn.1000-6362.2019.04.003 王雅静,张欣莹,黄桂荣,等.磷脂酶 D 参与冬小麦对干旱胁迫的响应[J].中国农业气象,2019,40(4):222-229 磷脂酶 D 参与冬小麦对干旱胁迫的响应* ** 王雅静，张欣莹，黄桂荣，封 富，钟秀丽 （中国农业科学院农业环境与可持续发展研究所/农业部旱作节水农业重点实验室，北京 100081 ） 摘要：磷脂酶 D （phospholipase D ，PLD ）通过水解细胞膜磷脂产生信使物质磷脂酸（PA ），并介导多种激素 与逆境的信号转导过程。为探讨 PLD 在参与干旱信号转导调控细胞膜稳定性方面的作用及其途径，采用在培 养液中加入 PEG-6000 模拟干旱胁迫，用 PLD 抑制剂正丁醇（n-butanol ，BA ）抑制 PLD 产生 PA ，对比 PEG+BA 处理与 PEG 处理下小麦抗旱性、膜稳定性以及抗氧化酶类的变化。结果表明，与 PEG 处理相比，PEG+BA 处理下，冬小麦幼苗叶片生长受抑制，净光合速率下降，光合非气孔限制作用增强，细胞膜离子渗漏率显著 升高，膜脂过氧化产物丙二醛（malonaldehyde ，MDA ）升高，抗氧化酶 POD 活性下降，表明 PLD 参与干旱 胁迫下过氧化物酶（peroxidase ，POD ）活性的调控。由此揭示出一条潜在的由 PLD 介导的干旱信号转导途 径，即干旱胁迫—PLD 激活—POD 活性增强—保护膜脂过氧化损伤—提高细胞膜稳定性—植物抗旱性增强。 关键词：磷脂酶 D ；干旱；信号转导；过氧化；抗氧化系统；PLD Phospholipase D of Winter Wheat is Involved in Responses to Drought Stress WANG Ya-jing, ZHANG Xin-ying, HUANG Gui-rong, FENG Fu, ZHONG Xiu-li (Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/ Key Laboratory of Dryland Agriculture, Ministry of Agriculture, Beijing 100081) Abstract: Phospholipase D (PLD) mediate signaling processes of various hormones and adversities by hydrolyzing phospholipids in cellular membrane and producing phosphotidic acid (PA), a messenger. In order to investigate the roles and pathways of PLD in regulation of the stability of cellular membrane, PEG-6000 was adopted to mimic drought stress, and n-butabol (BA) was applied to inhibit PLD-derived PA. Drought resistance, stability of cellular membrane, antioxidant enzymes of wheat seedlings treated by PEG and PEG added BA were compared. The results showed that inhibition