山竹子果壳提取物对离体肠及脑神经细胞离子通道的影响-生理学专业论文.docxVIP

山竹子果壳提取物对离体肠及脑神经细胞离子通道的影响 山竹子果壳提取物对离体肠及脑神经细胞 离子通道的影响 硕士研究生：黄勃 指导老师：项辉副教授 专业：生理学 摘 要 天然药物是医药的重要组成部分，是我国新药研究和开发的重点。山竹的果 壳被广泛用于治疗腹泻和疟疾、抗炎、抗溃疡以及抗白血病和败血病等，并且还 具有明显的抗过敏和平喘等多种用途。 实验采用大鼠离体肠段，置于装有台式液的恒温灌流浴槽中，记录肠段收缩 活动，探讨山竹子果壳提取物对消化道平滑肌的作用及其作用机理。结果表明， 山竹子果壳提取物对由乙酰胆碱和氯化钡引起的大鼠离体肠收缩有明显抑制作 用。 用全细胞膜片钳记录方式研究了山竹子果壳提取物对乳鼠脑神经细胞钾通 道的作用机理，对酶解分离的乳鼠脑神经细胞旄一跃迁电压可引出外向钾电流。 加入100ug／ml的山竹子果壳提取物后，外向钾电流明显减少。 结论：山竹子果壳提取物对离体肠及脑神经细胞具有一定生理药理作用。 关键词：天然药物，山竹子，离体肠，钾通道，全细胞膜片钳 Effect Effect of extracts from mangostana Oil the isolated ileum and brain neuron of rat Master candidate：Huang Qing Supervisor：Assistant Prof．Xiang Hui Major：Physiology Abstract Natural medicin is an important part of medicament，and is also the key of researching and invention of the new medicine in China．The hull of the mangostana is largely used in curing diarrhea，inflammation， canker,leukaemia，sapraemia，and it has obviously effect on curing allergy and asthma． The effect and mechanism of mangostana on contractile activity of isolated ileum segments in rats are observed．One end of all ileum segment is fixed in a tissue chamber while the other end is connected to an isometric force transducer，then motility of segments are recorded The results show that mangostana can obviously inhibit the contractile activity of ileum segments evoked by acetylcholine and barium chloride Whole‘。cell patch·。clamp recording mode was used to study the action II mechanism mechanism of mangostana’extracts on potassium channel currents of rat brain neuron．The outward K+current was activated by the depolarizing pulse in isolated rat brain neuron．Wherl lOOug／ml mangostana extracts was added to the bath solution，the outward K+ current was distinctly suppressed Conclusion：The hull of the mangostana has the physiological and pharmacological action on intestine and brain neuron Keywords：natural medicin，mangostana，isolated ileum，potassium channels，whole—cell patch·clamp m 第一章天然药物1．天然药物——医药的重要组成部分 第一章天然药物 1．天然药物——医药的重要组成部分 天然药物是从植物、动物和微生物等天然资源中开发出来的药物，是