榄香烯对肝癌细胞株中抑癌基因甲基化影响的实验研究-普通外科学专业论文.docxVIP

PAGE PAGE IV III III 中文摘要 榄香烯对肝癌细胞株中抑癌基因甲基化影响的实验研究 凋亡，并且阻滞两种肝癌细胞从 S 期进入 G2/M 期，呈浓度依赖性。 2.榄香烯具有逆转抑癌基因异常甲基化作用，但具有基因和细胞特异性。 3. GSTP1、RIZ1、HAI-2 基因甲基化状态具有很好的肝癌特异性，可建立甲基化 谱，可较好的区分肝癌与非肝癌。 关键词：榄香烯；抑癌基因；甲基化 研究生：黄洪军 指导老师：江勇 Experimental study of elemene on methylation of tumor suppressor genes in hepatocellular carcinoma cell lines Abstract Experimental study of elemene on methylation of tumor suppressor genes in hepatocellular carcinoma cell lines Abstract Objective: To study the effect of elemene on the proliferation, apoptosis and cell cycle of HepG2 and QGY7703 cells, To certain anti-tumor effect of elemene; To investigate the promoter methylation status of GSTP1, RIZ1, SLIT2, HAI-2 in elemene group and control group of hepatocellular carcinoma cells, explore whether elemene can reverse the hypermethylation of silenced TSG; To select the methylation profile for the early clinical diagnosis of HCC. Method: MTT assay was used to determine the growth inhibition of HepG2 and QGY7703 cells. Flow cytometry was performed to analyze the cell cycle distribution and apoptosis of HepG2 and QGY7703 cells. Methylation status of GSTP1, RIZ1, SLIT2, and HAI-2 genes promoter were detected by Methylation Specific PCR (MSP) in elemene group and control group of hepatic carcinoma cell Iines. Result: After elemene was added to HepG2 and QGY7703 for 48h, elemene could inhibit the proliferation, difference between the groups was statistically significant (P0.05), IC50 for both HepG2 and QGY7703 were (69 ± 6.3) μg/ml and (63 ± 2.1) μg/ml; Natural apoptosis of HepG2 and QGY7703 were (6.37±0.15) μg/ml and (7.40±0.66) μg/ml without treatment of elemene, the 10，20, 30 μg/ml different concentration of elemene could induce apoptosis, the apoptosis ratios were (20.83±0.25) μg/ml and(12.50±0.61) μg/ml, (27.7±0.75) μg/ml and (32.77±2.30) μg/ml, (37.1±0.46) μg/ml and (48.27±1.23) μg/ml，difference between the groups was statistically significant (P0.05); The 10，20, 30μg/ml different conc