超临界CO2体系下酶法制备中碳链甘三酯的研究-中国粮油学报.docVIP

超临界CO2体系下酶法制备中碳链甘三酯的研究 姜洋1 齐晓芬1 李丹1 王彤1 张博坤2 于殿宇1* (东北农业大学食品学院1，哈尔滨 150030) (哈尔滨普润油脂有限公司2，哈尔滨 150028) 摘 要 中碳链甘三酯作为一种低能量的油脂因其特殊的生理功能和代谢途径得到了广泛的应用，本文以甘油和辛酸、癸酸混合物为原料，研究在超临界CO2体系下酶法制备中碳链甘三酯的工艺条件，并对酯化产物进行分析。通过脂肪酶的比较，得出脂肪酶Novozyme 435优于Lipozyme RM IM，因此，选用脂肪酶Novozyme 435为催化剂，以中碳链甘三酯得率为指标，通过响应面法对工艺条件进行优化，得出最佳反应条件为：反应压力9.1 MPa、反应温度90℃、酶添加量4.2%、底物摩尔比3:1和反应时间10.2 h。在此反应条件下中碳链甘三酯的得率为95.1%，酯化率为98.62%。该方法有效的提高了脂肪酸的利用率及MCT得率，所得酯化产物色泽较浅。 关键词 超临界CO2体系 酶法 中碳链甘三酯 响应面优化 中图分类号：TS224.8 文献标识码：A 文章编号： The Study of Enzymatic Preparation of Medium-Chain Triglycerids in Supercritical CO2 System Jiang Yang1 Qi Xiaofen1 Li Dan1 Wang Tong1 Zhang Bokun2 Yu Dianyu1* (College of Food Science and Technology Northeast Agricultural University1,Harbin 150030) (Harbin PuRun Oil Co., Ltd.,Harbin 150028) Abstract The medium-chain triglycerids as a Low energy fat have been widely used for their special physiological functions and metabolic pathways.In this paper, glycerin and caprylic acid, capric acid mixture as a raw material,study of enzymatic preparation of medium-chain triglycerids in supercritical CO2 system conditions. By lipase preferably derived lipase Novozym 435 superior Lipozyme RM IM, therefore, selection lipase Novozym 435 as catalyst, medium-chain triglycerids yield as indicators, use response surface method to optimize the process conditions.The optimal reaction conditions for that:the pressure is 9.1 MPa, reaction temperature is 90℃, enzyme dosage is 4.2%, substrate molar ratio of 3:1 and time is 10.2 h. Under the reaction conditions,the medium-chain triglycerids yield is 95.1%,esterification rate is 98.62%. The method effectively improves the utilization of fatty acids and MCT yield. Key words Supercritical CO2 system, enzymatic, medium-chain triglycerids, response surface optimization 中碳链甘三酯（MCT）是指由C6～C12饱和脂肪酸所构成的甘油三酯，一般为辛酸甘三酯、癸酸甘三酯或辛酸-癸酸混酸组成的甘三酯[1,2]。大量的研究发现，高脂肪膳食与肥胖症、高血压、高血脂、脑血栓等疾病及某些癌症（乳腺癌、肠癌）发病率的上升存在着密切的关系[3-5]。因此，营养专家不断提倡低油、低糖、低盐、高纤维的“三低一高”饮食原则，而基金项目：