抗血管内皮生长因子抗体对肝癌的放射增敏作用(一).pdf

抗血管内皮生长因子抗体对肝癌的放射增敏作用 (一) 作者：郑青平 ,陈龙华 ,石玉生 【关键词】肝肿瘤 ;放射疗法 ;抗 VEGF单抗 ;放射增敏 Radiosensibilityofhumanhepatocellularcarcinomaxenograftsenhancedbyan tivascularendothelialgrowthfactormonoclonalantibody 【 Abstract 】 AIM:Toinvestigatetheinhibitingeffectsofcombiningantivascularendothelialg rowthfactor(VEGF)monoclonalantibody(mAb)therapywithradiotherapyont hegrowthofhepatocellularcarcinomaxenograftsinvivo.METHODS:Humanhe patocellularcarcinomacellline,SMMC7721,wasimplantedscinmice.Themice withxenograftsweredividedinto4groups,radiation(RT)20Gygroup,RT20Gypl usmAbgroup,RT30GyplusmAbgroupandcontrolgroup.AntiVEGFmAbwasinje ctediponalternatedaysforatotalof6injectionsatadosageof50 μ eradiationdosesweregiven24hafterthe4thinjectionofmAb.Tumordiameters weremeasuredandtumorvolumewascalculated.Miceweresacrificed2wkafte rthe6thinjectionandtheintratumoralmicrovesseldensitywasdeterminedbyc ountingthestainedvesselswithimmunohistochemistry.RESULTS:Radiationsig nificantlyreducedthegrowthandthemicrovesseldensityoftumors.Theinhibit oryeffectsweremoresignificantwhenradiationwascombinedwithantiVEGFm Ab,andRT30GyplusmAbwasmoreeffectivethanRT20GyplusmAb.ForRT20Gy group,RT20GyplusmAbgroupandRT30GyplusmAbgroup,theinhibitoryrateso ftumorweightwere75.3%,83.9%and94.7%,respectively.CONCLUSION:AntiV EGFmAbenhancestheradiosensibilityofhumanhepatocellularcarcinomaxen ografts.Thestrategyisoneoftheapproachesforhepatocellularcarcinomatreat ment. 【 Keywords 】 hepatocellularcarcinoma;radiotherapy;antiVEGFmAb;radiosensibility 【摘要】目的 :观察抗血管内皮生长因子抗体 (VEGFmAb)与不同剂量放 射联合对肝癌裸鼠移植瘤生长的抑制作用 .方法 :将 SMMC7721 肝癌细 胞种植于裸鼠皮下 ,成瘤动物分为单纯放射 20Gy组、放射 20Gy+抗体组、 放射 30Gy+抗体组和对照组 ,腹腔给予抗 VEGFmAb50μg/ 只，隔日 1 次, 共 6 次,放射剂量一次性给予 .测量肿瘤直