建立日本大白兔症状性脑血管痉挛模型及继发脑损伤研究.doc

兔症状性脑血管痉挛模型建立及继发脑损伤评估 莫云长 王均炉 周乐平 胡章勇 裴圣林 陈伟健 【摘要】 目的 建立日本大白兔症状性脑血管痉挛模型及继发脑损伤评估。方法 结扎双侧颈动脉结扎后枕大池二次注血的方法制成兔蛛网膜下腔出血( SAH)模型。实验分为2组, 实验组( 枕大池注血) 和假手术组( 枕大池注0.9%氯化钠)，观察SAH 前后动物进食量和神经功能改变,并在术前(day 0)、术后(day 5)行3D-CTA检查，之后断头取海马组织行电镜观察，并测定超氧化物歧化酶(SOD)活性 、丙二醛（MDA）含量。结果 与对照组对比，SAH 后动物进食量下降、神经功能障碍,3D-CTA测定SAH组兔基底动脉术后5天较术前痉挛程度为(27.12±11.82) % ，SAH组海马在电镜下可见神经元核膜皱缩，线粒体空泡样变，有髓神经脱髓鞘等神经损伤的改变。SAH组兔与对照组比较Day5时，SOD活性降低 ，MDA含量升高。结论 该模型可以作为蛛网膜下腔出血后症状行脑血管痉挛的实验模型,3D-CTA可有效判断脑血管痉挛的程度，海马组织SOD、MDA指标变化可评价继发脑损伤程度。 【关键词】　症状性脑血管痉挛　动物模型 继发脑损伤 Establishment rabbit model of symptomatic cerebral vasospasm and evaluation of secondary brain Injury MO yun-chang , WANG jun-lu ,ZHOU le-ping et al. Department of Anesthesiology, the First Affiliated Hospital of Wen Zhou Medical College , Wenzhou 325000, China 【Abstract】 Objective To establish japanese white rabbit model of symptomatic cerebral vasospasm and evaluation of secondary brain Injury. Methods After occlusion of bilaeral carotid 2 weeks, the survival rabbits were randomized to 2 groups: control group, subarachnoid hemorrhage (SAH) group. The animals of the subarachnoid hemorrhage group were injected with nonheparinized autologous arterial blood into cisterna magna, while the animals of control group were injected with sodium chloride. All of them were examined by 3D-CTA before the operation (day 0) and after the operation (day5). The hippocamps were removed from brains, observed by electron microscope, and measured the contents of SOD and MDA. Result Compared with the control group,food-intake, the neuro-logical functional disorder decreased in all animals ,and compared with preoperation, the severity of basilar arterious vasospasm assessed by 3D-CTA is (27.12±11.82) % after the fifth day . The nerve injury of neurons nuclear membrane invagination、mitochondria vascuolated and myelinoclasis of myelinated nerve was observed under electron microscope in the SAH group. Compared with the control group, SOD activit