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* * * * Standard line sizes to connect easily up to most bioreactors have been aligned with our SU flow paths. This ensures the pump is not being starved therefore provides a robust scaleable process without impacting cells. To summarize our systems….. * The KML50 and KML100 which can work with Membrane areas starting with 115cm2 and up to 1.0 m2 This one KML100 system alone can work with bioreactors starting from 1.5L working volume up to 100 liters where as the KML50 new in 2016 will work with bioreactors 1liter working volume up to 10 liters. The Onlly System modification required for scale up is the change out of the disposable flow path components to maintain volumetric throughput For the perfuse operaiton. The KPS platform….. * KPS 600 platform will manage SU Membrane asemblies with areas ranging from 0.51m2 to 12.8m2; Note KML-1000 provides additional pumping which we anticpate for running multiple filters simultaneously on larger reactors or under other circumstances like higher vessel volume exchanges which will require more hollow fibers in parallel to support the demand of VT for these operations. Note our typical flow path comes complete with…. * The KTF1000 platform will manage SU Membrane asemblies with areas ranging from 4.35m2 to 17.1m2; Designed specifically for our KrosFlo Max Module offerings in UF or MF applications with optional auxillary pumps. In addition the KTF-1000 can be designed for daisy chained systems to provide additional pumps for running multiple filters simultaneously on larger process’s or under other circumstances like higher vessel volume exchanges which will require more hollow fibers in parallel to support the demand of VT for these operations. Note our typical flow path comes complete with…. * * * * * * 90年代已上市产品中选择重力的较多,但是目前已经很少有人开发相关工艺 * 已上市产品基本没有使用 * * 老工艺,已上市老产品有使用 * * * * * * * * * 抑制细胞生长的方法包括降低温度和添加化学物质(如丁酸盐),但前提是保证细胞特异性产率和产物质量。另外,降低温度的另一个优势是,蛋白水解酶的活性也降低了 低补液、低废弃,可导致营养限制 高补液、低废弃,无营养限制,提高废弃,可提高活性
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