羽叶鬼灯檠的化学成分及活性研究和两种生物活性筛选模型的应用研究.docx

摘 要 10μg/ml and 12.5μg/ml, respectively, in which two compounds 2001146 and 200111132 showed significant activities with IC50 at 0.30 and 0.37μg/ml, and extract 2001034 IC50 at 0.74μg/ml, respectively. Chapter 4 Progress in the Screening of Depigment Drugs Pigmentation is an important issue nowadays. In this chapter the progress of depigment drugs R D including melanogenesis pathway, methods in screening depigment drugs, class of depigment and tyrosinase inhibitors as well as their inhibitory mechanism were reviewed. Key words: Rodgersia pinnata, Saxifragaceae, Chemical Constituents, Active Constituents, Bioassay, Depigmentation, Tyrosinase, Anti-osteoporosis, Carbonic anhydrase II. V 羽叶鬼灯檠的化学成分及活性研究和两种生物活性筛选模型的应用研究 Compounds from Rodgersia pinnata Franch. RO OH O O OH O OH CH3 OH OH OH OH 1* R = α-L-rhamnopyranosyl α-L-rhamnopyranosyl 2 R = α-L-arabinofuranosyl RO O O O OH OH OH OH OH OH 3 R = α-L-arabinofuranosyl β-D-xylopyranosyl 4 R = α-L-rhamnopyranosyl R = β-D-xylopyranosyl R = α-L-arabinopyranosyl COOH RO HO 7* R = OH O  O OH HO OH α-L-arabinofuranosyl OH O OH OH α-L-arabinopyranosyl COOH 8 OH OH OH H3CO OH OH OH HO O O OH HO O O OH HO OH OCH3 HO OCH3 OH 9* 11 10* 12 VI 化合物结构 OH OR2 13R1=R2=H HO O 11 OH 14R1=G,R2=H OH O 2 OH 15R1=H,R2=G H O MeO 10 10b4a 4 OH OH O 10a OR1 H O 6 O HO G= OH HO O 16 7 O OH HO OH OH OH OH OH HO O HO O OH O O OH OH OH 17 18 HO OH OH OG OH HO O OG HO O HOOC OH OH HO HO OH OH OH 19 20 22 21 CH3(CH2)nCOOH HO 25 n = 14 GluO 26 n = 16 23 24 27 n = 24 VII 第一章 羽叶鬼灯檠的化学成分及活性研究 第一章 羽叶鬼灯檠的化学成分及活性研究 1.1 引言 羽叶鬼灯檠（Rodgersia pinnata Franch.）为虎耳草科（Saxifragaceae）鬼灯 檠属植物，从世界范围论，属东亚分布类型，生于海拔 700－3800m 的林下、林 缘、灌丛、高山草甸和石隙。本属植物经考订认为只有 5 种和 3 变种，日本、朝 鲜、锡金、尼泊尔仅各产一种，而我国产 4 种和 3 变种（其中有 3 种和 3 变种为 我国特有），在虎耳草科中为较小的属。按植物区系可将该属划分为 4 个分布类 型：（一）日本－朝鲜间断分布；（二）秦岭－大巴山分布；（三）横断山分布； （四）东喜马拉雅分布。横断山地区即中国西南地区为该属现代分布中心和分化 中心[1]。 羽叶鬼灯檠又叫九叶岩陀、岩陀、红姜等，主要分布于中国西南地区云南、 四川两省，海拔 2200－3900m 的山丘溪边、林下阴湿处。味苦涩、微甘，性凉， 具有活血调经、行气、祛风湿、收敛、消炎止痛、健胃止泻等功效，主治跌打损 伤、接骨、劳伤咳嗽、月经不调等，功效与西南鬼灯檠（R. sa