CTGF与TGFβ 1在先天性心脏病心肌重整塑中的表达及相关研究.docxVIP

优秀毕业论文 精品参考文献资料 硕士学位论文 硕士学位论文 中文摘要 表达及缺损大小成正相关(CTGF与I型胶原蛋白r=O．559，pO．001； CTGF与III型胶原蛋白r=O．331，pO．05；CTGF与缺损大d、r=O．563， pO．001；TGF—B l与I型胶原蛋白r=0．325，pO．05；TGF—B l与III型 胶原蛋白r=O．403，pO．05；TGF—B 1与缺损大d、r=O．416，pO．05)， 与血氧饱和度成负相关(CTGF与血氧饱和度r=-O．744，pO．001；TGF- B 1与血氧饱和度r一0．631，pO．001)；(5)CTGF与TGF—B 1mRNA两 者的表达水平存在相关性(r=O．616，pO．001)。 结论(1)紫绀型先天性心脏病较非紫绀型先天性心脏病患儿 心肌纤维化明显；(2)CTGF与TGF—B。在紫绀型先天性心脏病患儿心 肌中显著增高，并与心肌纤维化指标存在显著正相关， CTGF及TGF- B 1可能参与了先天性心脏病特别是紫绀型先天性心脏病心肌纤维 化的过程。(3)先天性心脏病患儿心肌纤维化可能同患儿年龄、缺氧 程度有关。(4)CTGF与TGF-B。的表达与血氧饱和度存在明显负相关， 与缺损大小存在正相关，提示缺氧及容量负荷增加可能会引起两者表 达增强。 关键词先天性心脏病，紫绀型先天性心脏病，心肌重塑，心肌 纤维化，结缔组织生长因子，转化生长因子一B 1 II 硕士学位论文 硕士学位论文 英语摘要 ABSTRACT， obj eetives To explore the expression of transforming growth factor—beta l(TGF·13,)and connective tissue growth factor(CTGF)in children with congenital heart disease(CHD)and analysis the relationship of TGF-p l and CTGF with the type of CHD(acyanotic congenital heart disease and cyanotic congenital heart disease)，age，and the oxygen saturation．To investigate roles of TGF—p 1 and CTGF in the development of myocardial fibrosis in CHD． Methods Of 45 childeren having CHD，25 were acyanotic congenital heart disease and 20 were cyanotic congenital heart disease．A small amount of fresh myocardium was cut from the right atrial appendage．To explore the expression oftype I，type III collagen，TGF-pl and CTGFmRNA through reverse transcription-polymerase chain reaction(RT—PCR)．SPSS 1 3．0 software was used for the statistical analysis of these data． Results(1)The myocardial fibrosis of cyanotic congenital heart disease was more significant than acyanotic congenital heart disease (type I collagen p0．01；type III collagen p0．01)．(2)The expression of TGF—p l and CTGF mRNA were higher in cyanotic congenital heart disease than acyanotic congenital heart disease(p0．00 1)。(3)The expression of type I,type III collagen mRNA were correlat