BSAPLGA缓甸释微球的制备研究.docxVIP

优秀毕业论文 精品参考文献资料 河北科技大学硕士学位论文 1I Abstract Abstract ：：=；：：： ：=：：=：=；====================；============================ Ab stract In recent years，with therapid deverloping of modem bioteehnolo鄹；genomies,and the combinatorial chemistry,polypeptide and protein drugs had been used in the treatment of human diseases．However,because this kind of drugs had high quality,short half-life， frequent injection dosage,and low bioavailability,studies on the drug delivery system had appealed scholars’attention．At present,to overcome these questions,biodegradable polymer materials was made into microspheres，and peptides were encapsulated in these microspheres．This method maintained the stability of the dn玛，got the slow release profile in the body,and finally improved drug bioavailability． In this paper,the coaeervation method was used to prepare BSA／PLGA mierospheres． We studied the influence factors on microspheres’preparation，such as the concentration of PLGA，molecular weight of PLGA，Protein protective agent dosage，theoretical drug loadings，the internal phase volume，the dose of coagulant,eolostrum u]旬r,Lsonic process， the condensation process，hardening solvent,hardening temperature，and drying technology．The evaluation indexes were particle size，surface morphology，and drug encapsulation efficiency,etc．Finally，we determined the optimal preparation procedure based on these evaluation indexes．The optimal procedure Was shown as followed：(1) PLGA concentration：80 mg／mL，the theory of drug loadings：3％，the dosage of sugar： 1．5‰the internal phase volume：100 pL，and silicone oil consumption：4 mL；(2)the colostrum ultrasonic technology：the ultrasonic intensity：300彤and ultrasonic time：1 0 min；(3)the condensation and hardening process：stirring temperature：1 5。C，stirring speed：500 rpm，and hardening solvent：heptane in 3 oC；(4)drying process parameters： vacuum drying，8 h 3 oc，2 h up to 25 oC，10 h 25 oC．With the optimal process，we fabricated BSA／PLGA microspheres．From the characterization，we could draw