脐带血清在骨髓造血祖细胞培养中的效应.docVIP

???? 脐带血清在骨髓造血祖细胞培养中的效应 ???? ????? 摘要 目的：探讨人脐带血清（CBS）在骨髓造血祖细胞培养中的效应。方法：用人骨髓细胞进行CFU-GM、CFU-E、BFU-E、CFU-GEMM培养。结果：CBS能直接刺激 骨髓细胞CFU-GM的形成。与血型相同否无关。四人份以上的混合CBS(MCBS)，刺激活性高且 稳定。10％MCBS相当于65．6 μg／L GM-CSF、0．23、0．3、0．46kU／L EpO对CFU-GM 、CFU-E、BFU-E、CFU-GEMM所起的作用。MCBS可提高CFU-GM、CFU-E、BFU-E、CF U-GEMM常规体系的集落产率。在CFU-GM培养时，20％MCBS与100 μg／L GM-CSF和 20％马血清具有相同的效果。MCBS可直接刺激CFU-E形成。如培养体系内无B pA存在时，形 成BFU-E、CFU-GEMM较少或几乎为零，当含BpA时，可形成一定量的BFU-E、CFU-GEM M集落。结论：CBS(MCBS)内：①含有GM-CSF、Epo等物质或因素；②具有刺激CFU-GM、 CFU-E、BFU-E、CFU-GEMM的生长活性；③同血型或不同血型4人份以上混合，刺激活性高 且稳定。具有良好的应用前景。关键词：　脐带血清；　骨髓；　造血祖细胞中分类号：　R331.1-33文献标识码：A　　 　　文章编号：1000-6834(2000)01-0068-04 EFFECTS OF CORD BLOOD SERUM ON COLONY CULTURE OF BONE MARROW HEMATOPOIETIC PROGENITOR CELLS HUANG You-zhang, LUO Hui, YANG Ping-di, SHEN Jian-liang(Department of Hematology, General Hospital of Navy, Beijing 10003) ABSTRACT　　Aim: To provide experimental proofs for Cord Blood clinical appliance, t he effects of human CBS (Cord Blood Serum) on colony culture of bone marrow hema topoietic progenitor cells were studied. Methods: Observed the colony forming capacity of CFU-GM，CFU-E，BFU-E，CFU-GEMM when they were c ultivated using hBMNC with CBS, Mixed Cord Blood Serum (MCBS) and/or cytokines. Resutts: 10% CBS could promote the forming of CFU-GM of BMC dir ectly, no matter what blood group the CBS or BMC were of. If four or more copies of CBS were mixed (MCBS), no matter whether the “ABO” blood groups between t hem were same or not, they could produce more CFU-GM than single copy or two-copi es-mixed CBS when used, and their activity was stable. MCBS could increase the production of CFU-GM、CFU-E、BFU-E、CFU-GEMM in routine culture system, and 10%MCBS corresponds with 65.6 μg/L GM-CSF，0.23，0. 3，0.46 kU/L Epo to CFU-G M ，CFU-E，BFU-E，CFU-GEMM respectively. When used in colony culture of CFU-GM ,20% MCBS has the same effect as 100 μg/L GM-CSF with 20% HS. 20% MCBS could a lso directly promote the forming of CFU-E. It formed few or none BFU-E and CFU -GEMM w