陆地棉基因GhLDOX和Gh4CL3的功能解析.pdfVIP

Functional studies of GhLDOX Gh4CL3 genes in upland cotton Gossypium hirsutum Table of Contents LIST OF ABBRIVATIONS VII Chapter I The leucoanthocyanidins dioxygenase (GhLDOX) gene regulates the formation elongation of cotton trichomes and fiber via modulating auxin signaling pathway…viii 摘要 i ABSTRACT iv 1.1 INTRODUCTION AND LITERATURE REVIEW 1 1.1.1 Cotton as an Economical Crop 1 1.1.2 Plant Trichomes are Unique Differentiated Cells 1 Transcriptional Regulation Underlying Leaf-Trichomes Development 2 Associated Hormonal Mechanisms Underlying Trichome Development in Arabidopsis .. 5 1.1.3 Cotton Fiber Development 5 Cotton Fiber Transcriptional Regulation 6 Role of Hormones in Regulating Cotton Fiber Development 9 1.1.4 Flavonoids 11 The Importance of Flavonoids to Plants 11 Flavonoids: Defensive Role in Cotton 11 Flavonoids are Responsible of Cotton Leaf Reddening 13 Flavonoids Function in Cotton Fiber Development 13 1.1.5 Anthocyanin Synthesis 14 Ldox: A Late Gene in Anthocyanin Biosynthetic Pathway 15 Role of Anthocyanins Coupled With Phytohormones in Cotton Fiber Development 16 Anthocyanins and Trichomes 18 Sugar/Light- Mediated Anthocyanin Accumulation 19 Anthocyanin Is a Buffer Zone to Protect Chlorophyll 21 1.1.6 CRISPR-Cas9 A Promising Tool for Plant Functional Genomics 22 1.1.7 Aim of Study 23 1.2. MATERIALS AND METHODS 24 1.2.1 Construction of Phylogenetic Tree and Digital Expression Profiling for GhLDOX 24 1.2.2 Specifi