生物化学资料：Manuscript-原核生物和真核生物的DNA复制1.0.pptVIP

模板来自于 / * 模板来自于 * 模板来自于 * 复制的终止与端粒酶 染色体DNA呈线状，复制在末端停止。 复制中冈崎片段的连接，复制子之间的连接。 染色体两端DNA子链上最后复制的RNA引物，去除后留下空隙。 * 复制的终止与端粒酶 染色体DNA呈线状，复制在末端停止。 复制中冈崎片段的连接，复制子之间的连接。 染色体两端DNA子链上最后复制的RNA引物，去除后留下空隙。 * * 端粒酶催化作用的爬行模型 端粒 真核生物的DNA 端粒 端粒重复序列 去除了终端的RNA引物的新链 1 端粒酶延长DNA的3端 新的端粒重复序列 端粒酶 2 DNA聚合酶α的引发酶亚基合成RNA引物 RNA模板是端粒酶的核糖核蛋白的一部分 3 DNA聚合酶α延长引物的3端 DNA聚合酶α发挥引发酶活性 DNA聚合酶α发挥聚合酶活性 RNA引物被移除 端粒酶延长DNA的3端 5’ 3’ ori ori ori ori 5’ 3’ 5’ 5’ 3’ 3’ 5’ 5’ 3’ replicon 3’ Eukaryotic DNA replicates as multiple replicons. 1. Multiple replicons: DNA replication 2. DNA replication related to cell cycle: When it comes to cell cycle... CDK cyclin CKI DNA replication initiation and pre-replicative complex (pre-RC) Origin recognition complex(ORC) Autonomously Replication Sequence(ARS) cyclinA-Cdk complex cyclinA-Cdk complex 3.Enzymes and proteins CDK Related cyclin Function period CDK2 Cyclin D1, D2, D3 G1 Cyclin E G1→S Cyclin A S CDK3和CDK4 Cyclin D1, D2, D3 G2 CDK1（CDC2） Cyclin A, B G2→M CNA Proliferation cell nuclear antigen P has the same function and a similar conformation as β subunit of E.coli DNA polymerase Ⅲ ,to form a sliding DNA clamp. Extending eukaryotic DNA replication Consisting of the end of DNA sequence and protein. GC-rich. shorten with each replication. 4. Telomere replication(Termination): Telomere TTTTGGGGTTTTGGGG… Telomerase Structure The creep model in the catalysis of telomerase Maintaining the structural integrity of the chromosome. Maintaining the integrity of DNA replication. The replication of telomeres Telomeres Telomerase extend the 3’-end The synthetic of RNA primers Extend the 3’-end of the RNA strand Excision of RNA primers Contrasts Prokaryotic DNA replication Eukaryotic DNA replication Origin of replication a oriC multiple ARSs Copy unit a primasome multiple replicons Primer long,many short,few Period of Replication during the replication cycle S Period DNA polymerases pol I,pol II,pol Ⅲ pol α，pol β，pol γ，pol ζ，pol