报告兽医专业英语.ppt

Immunomodulatory effect of swine CCL20 chemokine in DNA vaccination against CSFV 郑仲华 微生物学 猪CCL20趋化因子在抗猪瘟病毒的 DNA疫苗中的免疫调节作用 1.Abstract The objective of this work was to explore whether a plasmid expressing CCL20 chemokine could improve the immune response against CSFV in co-administration with a DNA vaccine expressing the E2 protein. The immunization of pigs with the DNA vaccine formulation,that contains swine CCL20 chemokine, resulted in the homogenous induction of detectable levels of CSFV antibodies at 36 days after the ?rst injection. 这项工作的目的是探讨CCL20 趋化因子质粒表达是否可以提高DNA 疫苗表达E2 蛋白联合控制加强对猪瘟病毒的免疫应答。免疫猪的 DNA 疫苗配方包含猪 CCL20 趋化因子，结果在疫苗第一次注射后的第36 天检测到同源诱导的猪瘟病毒的抗体水平。 .精品课件. * Remarkably, immunized animals with E2 DNA vaccine in co-administration with the plasmid containing swine CCL20 developed high titers of neutralizing antibodies against homologous and heterologous（同源和异源）CSFV strains and were totally protected upon a lethal viral challenge (sterilizing protection). 引人注意的是，E2 DNA 疫苗和含猪 CCL20 质粒共同作用下的免疫动物，在致死量病毒的作用（减毒保护）下产生对同源及异源猪瘟病毒株高效价的中和抗体，并使之得到完全保护。这证实 CCL20 具有增加抗体免疫反应的作用。 .精品课件. * 2.Introduction It is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils（中性白细胞）. CCL20 is implicated in the formation and function of mucosal lymphoid tissues（粘膜淋巴组织） via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells（上皮细胞） surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6. What is CCL20？ .精品课件. * Classical swine fever virus is the causative agent（病原体） of classical swine fever (CSF), one of the most important swine infectious diseases. CSFV is an icosahedral(二十面的) RNA virus with (+) polarity（正极） and a lipid envelope（脂质包膜） that belongs to the Pestivirus Genus（瘟病毒属）of the Flaviviridae family（黄病毒科）. Then,lets briefly know CSFV. .精品课件. * 3.Mater