不同碳源和光照对小球藻生长及产油脂的影响汇总.docxVIP

南昌大学 硕士学位论文 不同碳源和光照对小球藻生长及产油脂的影响 姓名：刘香华 申请学位级别：硕士 专业：环境工程 指导教师：刘雷 PAGE PAGE # PAGE PAGE # fill 生物柴油因其具有与石化柴油相近的理化性质，且生物柴油是一种可生物 降解、清洁、环境友好和安全的可再生性环保新能源，这使它成为21世纪能源 研究的新宠。微藻油脂是生物柴油的制备原料之一，微藻具有易于培养、光合 利用效率高、生物周期短和油脂含量高等优点使得微藻油脂的研究成为生物柴 油研究的热点。本文以自行筛选的小球藻（chloreHavugaris）为研究对象，为了 优化小球藻的生长和产油条件以快速的获得高生物量和高油脂产量。本文考察 了 3种碳源（葡萄糖、NaHC03和醋酸钠）及其不同的初始碳源质量浓度和光照 条件对小球藻（a/。%乐w/garis）生长和产油脂的影响,分析实验结果得出结论如 下： 3种碳源中前萄糖对小球藻的生长有显著的促进作用，葡萄糖是最优 搂源，低浓度的NaHC03能加速小球藻的生长，过高浓度的NaHCCh抑制小球 藻的生长，醋酸钠不利于小球藻的生长，尤其是混养条件下会加速藻细胞死亡; 3种光照条件下，藻细胞的生长速率随光照的增强而提高，16001X的 弱光照更利于油脂的积累，油脂含量在3种光照强度下表现为16001x50001x0 lx； （3）在设定的各种培养条件下，得出小球藻最佳生长和产油条件为：5000LX 光照，15g/L的葡萄糖作为外加碳源，获得了 3.17g/L的最大生物量和L025g/L 的最大油脂产量。 结合比生长速率和油脂含量这两个参考因素，得出更经济的小球藻生 长和产油条件为：50001x光照强度,5g/L的葡萄糖作为外加碳源,获得了 2.793睚 的最大生物量和1.012g/L的最大油脂产量。 关键词：小球藻；碳源；光照条件；微藻油脂 ABSTRACT ABSTRACT PAGE PAGE # ABSTRACT ABSTRACT PAGE PAGE # ABSTRACT I I The physical and chemical properties of biodiesel is close to petrochemical diesel, and biodiesel is a kind of renewable energy sources which have some advantages like biodegradable, clean, environmental friendly and safety, which make it to be the newly bestowed favor on the energy research at 21st century. Microalgac I oil is one of the promising raw material for the production of biodiesel, and microalgae has much advantage such as it is easy to cultivate, it have high I photosynthetic efficiency, its biological cycle is short and its fatty acid content is much higher than other oil plants and so on, this advantages make the research of I microalgae oil as the hot point in the biodiesel researches. This paper focused on the screening chlorella vugaris and takes it as the research object, in order to search best I culture and product oil conditions of chlorella vugaris fbr get great biomass and high lipid production quickly. We studied the effects of three carbon sources, i.e. glucose, I NaHCOj and sodium acetate, we have also studied the effects of their respective initial concentrat