川芎嗪对BMT后小鼠骨髓基质细胞bFGF表达水平的影响.docVIP

川芎嗪对BMT后小鼠骨髓基质细胞bFGF表达水平的影响 正文 川芎嗪对BMT后小鼠骨髓基质细胞bFGF表达水平的影响 文1：川芎嗪对BMT后小鼠骨髓基质细胞bFGF表达水平的影响 Effect of Ligustrazine on the Expression of bFGF in bone marrow stromal cells of mice after BMT AbstractThis study was purposed to investigate the effect of ligustrazine on the expression of bFGF in bone marrow stromal cells (BMSC) and to explore the mechanism of hematopoietic recotitution after bone marrow traplantation (BMT). The mice were randomly divided into 3 groups： normal group,saline group and ligustrazine group. BMT mouse models were established. The mice of normal group were not treated, the mice of saline group were given normal saline ( ml/mouse, twice a day) through gastric tube, while the mice of ligustrazine group were given ligustrazine ( ml/mouse, twice a day) through gastric tube. On day 7, 14, 21 and 28 after BMT, the femora were taken and the bone marrow mononuclear cell (BMMNC) suspeio were used for the cultivation of bone marrow stromal cells according to Dexters culture method. The mRNA and protein expressio of bFGF in BMSC were assayed by RT-PCR and Western blot respectively. The results showed that the expression of bFGF in BMSC on the level of mRNA and protein were all reduced significantly after BMT, and increased slowly with the time. On day 7,14 and 21 after BMT, the expressio of bFGF mRNA and protein in bone marrow stromal cells of ligustrazine group and saline group were lower than that in bone marrow stromal cells of normal group, but the expressio of bFGF mRNA and protein in ligustrazine group were obvioly higher than that in saline group (P or P). On day 28 after BMT, the expressio of bFGF mRNA and protein in ligustrazine group returned to normal level, while the expressio of bFGF mRNA and protin in saline group not returned to normal level, there was significant difference between these two groups. It is concluded that ligustrazine can enhance bFGF expression level in bone marrow stromal cells after syngeneic bone marrow traplatation in mice,