成纤维细胞生长因子受体3在脂多糖抑制成骨细胞分化中作用的初步研究临床医学.docVIP

成纤维细胞生长因子受体3在脂多糖抑制成骨细胞分化中作用的初步研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：成纤维细胞生长因子受体3在脂多糖抑制成骨细胞分化中作用的初步研究临床医学 2 1 动物分组 3 2 细胞培养 3 3 定量聚合酶链式反应(PCR) 4 4 碱性磷酸酶(ALP)活性检测 4 5 统计分析 5 1 FGFR3在LPS引起的炎症反应中的作用 5 2 FGFR3在LPS引起的成骨细胞分化抑制中的作用 5 文2：抗氧化剂Ｕ７４３８９Ｇ在高氧肺损伤中作用的研究进展临床医学 7 1 高氧性肺损伤的机制 8 2 U 74389 G的抗氧化作用途径 9 3 小结 12 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 成纤维细胞生长因子受体3在脂多糖抑制成骨细胞分化中作用的初步研究临床医学 文1：成纤维细胞生长因子受体3在脂多糖抑制成骨细胞分化中作用的初步研究临床医学 Abstract： Objective This experiment focuses on the differentiation of osteoblasts in the endotoximia while the FGFR3 pathway is activating,so as to undetand the change of bone tissue in Six male C57 mice and 6 male Ach mice (peistently activating function of FGFR3),8 weeks old,were divided into normal saline() group and lipopolysaccharide(LPS) group,3 mice per was extracted from the right tibias 4 hou after LPS or physiological saline injection,and then the levels of interleukin 6(IL6),tumor necrosis factor α(TNFα),osteocalcin (OC) in tibia and osteoblast were measured by quantitative realtime hou after the preosteoblast cell line MC3T3E1 was cultured with LPS and bFGF,the levels of IL6,TNFα,OC in tibia and osteoblast also were measured by quantitative realtime MC3T3E1 activities of alkaline phosphatase(ALP) were detected 7 days after Both of the levels of IL6 and TNFα in tibias and MC3T3E1 were increased while the levels of OC in tibias and MC3T3E1 were decreased 4 hou after LPS administration(P).The expression change was more huge in Ach mice(P).The expressio of IL6,IL1 and OC in MC3T3E1 cultured with LPS and bFGF were higher than those cultured only with LPS and lower than those only with with the untreated group,the activity of ALP in MC3T3E1 was decreased in LPS group(P) and increased in bFGF group(P).Conclusion LPS could involve bone tissue in inflammation and inhibit the differentiation of activating the function of FGFR3 in vivo could aggravate these low dose of bFGF in vit