糖尿病大鼠来源骨髓间充质干细胞体外培养观察及其生物学特性（临床医学资料）.docVIP

糖尿病大鼠来源骨髓间充质干细胞体外培养观察及其生物学特性（临床医学资料） 文档信息 属性： F-01NWGC，doc格式，正文26856字。质优实惠，欢迎下载！ 适用： 作为医学资料、临床医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：糖尿病大鼠来源骨髓间充质干细胞体外培养观察及其生物学特性 2 1 材料和方法 3 2 结果 5 3 讨论 7 文2：SLE患者骨髓间充质干细胞生物学特性的研究 9 1 资料与方法 9 2 结果 10 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 糖尿病大鼠来源骨髓间充质干细胞体外培养观察及其生物学特性（临床医学资料） 文1：糖尿病大鼠来源骨髓间充质干细胞体外培养观察及其生物学特性 Abstract: Objective: To study the effect of diabetic inner environment on the biological properties of marrow mesenchymal stem cells derived from bone(BMSCs). Methods: BMSCs were harvested from the normal and diabetic rats induced by streptozotocin (STZ). Cell proliferation was evaluated using cell counting kit-8 (CCK-8) assay. The secretory volume of vascular endothelial growth factor (VEGF) and iulin-like growth factor (IGF-1) were measured by enzyme-linked immunosorbent assay (ELISA) and the anti-apoptosis capacity of the cell under the hypoxia and serum deprivation (hypoxiaD) was detected by Hoechst33342 staining and annexin V/PI dual-color flow cytometry. Results: BMSCs from the diabetic rats induced by STZ could be successfully harvested and expanded in vitro. However they showed more spread-out and flattened appearance and larger size. The proliferative ability of diabetic rats derived from BMSCs were significantly decreased compared with the normal rats (P ). In addition， similar results were also presented in the cytokines (VEGF and IGF-1) release （± vs ± pg/mL P ，± vs ± pg/mL P ，respectively）and the ability of anti-apoptosis under hypoxiaD (P ). Conclusion: BMSCs derived from diabtetic rats can be successfully cultured in vitro. The ability of series of a biological properties in proliferation， paracrine and anti-apoptosis have been impaired in different degrees. Key words: diabetes；mesenchymal stem cells；vascular endothelial growth factor；apoptosis；proliferation；rats 干细胞 治疗 心肌梗死已被证实是一种较有前景的治疗方法。骨髓间充质干细胞（bone mar-row mesenchymal stem ce