偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学浅析.docVIP

偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学浅析 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学浅析 1 1 实验材料 3 2 实验方法 3 3 结果 6 2组大鼠脾脏淋巴细胞2-DE图谱及差异蛋白质点分析 6 4 讨论 7 文2：天麻钩藤饮加减治疗肝阳上亢型偏头痛 9 1 资料与方法 9 2 结果 10 3 讨论 11 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学浅析 文1：偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学浅析 Comparative Proteomics Analysis on Splenic Lymphocytes in Migraine Rat Model with Hyperactivity of Liver-yang HU Jian-jun， CHEN Ze-qi， ZHONG Guang-wei， et al Xiangya Hospital Affiliated to Central South Univeity， Changsha 410008， China　　Abstract：Objective To explore the molecule mechanisms of migraine with hyperactivity of liver-yang related protei. Methods Animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation， and by administering orally with Fuzi decoction. The total protei of splenic lymphocytes in the rats were separated by immobilized pH gradient-based two-dimeional gel electrophoresis. With coomassie blue staining， the 2-DE images were analyzed by PDQuest software. Matrix-Assisted Laser DesorptioIonization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) and database were used to identify the differential protei. Result Ten differential spots protein were identified， and they were chloride intracellular channel 1， thioredoxin peroxidase Ⅰ， thioredoxin peroxidase Ⅱ， sequence 7 from patent WO， Rho GDP dissociation inhibitor (GDI) alpha， actin related protein 2/3 complex neurofilament light polypeptide， proteasome subunit beta type and heat shock protein-27， annexin A1. Conclusion These protein may be involved in the development of migraine with hyperactivity of liver-yang. These data provide useful clues for elucidating the mechanisms of migraine with hyperactivity of liver-yang。 Key words：proteomics；migraine；hyperactivity of liver-yang；splenic lymphocytes；rat 偏头痛(migraine)在以头痛为症状的疾病中占有很重要的地位，在美国，约有%的女性和6%的男性曾有偏头痛发作[1]。本病2/3患者为单侧头痛，1/3为双侧，每次发作延续数小时或数天，