家畜细胞遗传图集.pdfVIP

家畜細胞遺傳 朱有田 References: 1. Lodish et al. 2000. Molecular cell biology (4th edition). W. H. Freeman and company. New York. 2. Hartwell et al. 2000. Genetics: from genes to genomes. McGraw-Hill co. New York. 3. Robertson. 1987. Teratocarcinomas and embryonic stem cells. IRL Press. Oxford. England. 4. Rooney. 2001. Human cytogenetics: constitutional analysis. Oxford university press. New York Dolly Dollay and cal Classical Genetics = Gregor Mendel (1865) 1: Simple organism: garden pea 2: Repeat self-pollination … Bred true 3: The trait could be determined unambiguously 4. Analysis of large number of descendant of his experimental crosses Dominant and Recessive Checkpoint Tumor formation 1/100- 1/10000 Embryonic stem (ES) cells The culture of embryonal carcinoma (EC) cells - the stem cells of teratocarcinomas Fluorescence Formation of ES cells carrying a knockout mutation General procedure for producing gene-targeted knockout mice Dolly 0.5 h cytokinesis Eukaryotic cell cycle 4.5 h Postmitotic cells: “exit” the cell cycle G0. Neuron 9 h Restriction point the point in late G1 where passage through 10 h the cell cycle become independent of mitogens Most bacterial chromosome are circular wit