桑黄提取物体内抗肿瘤作用的实验研究.docxVIP

桑黄提取物体内抗肿瘤作用的实验研究 　　[摘要]目的通过检测桑黄3种提取物对h22荷瘤小鼠的抑瘤率，探讨桑黄提取物的抗肿瘤活性。方法将spf级昆明种小鼠随机分组后，于小鼠右前肢腋窝皮下接种h22肝癌细胞，接种1d后，分别采用桑黄3种提取物的低、中、高剂量（100、500、1000mg·kg-1·d-1）灌胃，以生理盐水和环磷酰胺为阴性和阳性对照，连续给药10d。实验期间每天测定各组小鼠的体质量;给药10d后处死小鼠测瘤质量，并计算各组抑瘤率。结果桑黄3种提取物可以增加h22荷瘤小鼠体质量。低、中、高剂量桑黄菌丝体多糖提取物对h22荷瘤小鼠的抑瘤率分别为32.87%、57.08%和68.35%;低、中、高剂量桑黄子实体多糖提取物对h22荷瘤小鼠的抑瘤率分别为44.14%、52.81%和73.69%;低、中、高剂量桑黄发酵液多糖提取物对h22荷瘤小鼠的抑瘤率分别为49.16%、50.63%和51.81%。结论桑黄3种提取物均具有较强的抗肿瘤活性，其中抗肿瘤效果最好的是桑黄子实体多糖提取物，该提取物也可以显著增加h22荷瘤小鼠体质量。 　　[关键词]桑黄;中草药;肝肿瘤;抗肿瘤药 　　[abstract]objectivetoinvestigatetheantitumoractivityofphellinusigniariusextractsbyanalyzingthetumorinhibitionrateofthreephellinusigniariusextractsinh22tumor`-bearingmice.methodsspecificpathogen`-freekunmingmicewererandomlydividedintogroupsandtheninoculatedwithh22hepatomacellsundertheaxillaoftherightforelimb.onday1afterinoculation，threephellinusigniariusextractswereadministeredbygavageatalowdoseof100mg·kg-1·d-1，amiddledoseof500mg·kg-1·d-1，andahighdoseof1000mg·kg-1·d-1;themiceinthenegativecontrolgroupweregivennormalsaline，andthoseinthepositivecontrolgroupweregivencyclophosphamide.thecourseoftreatmentwas10consecutivedaysforallgroups.bodyweightwasmeasuredeveryday，andafter10dofadministration，themiceweresacrificedtomeasuretumorweight，andtumorinhibitionratewascalculatedforallgroups.resultsthesethreephellinusigniariusextractsincreasedthebodyweightofh22tumor`-bearingmice.thelow`-，middle`-，andhigh`-dosemyceliapolysaccharidesfromphellinusigniariushadatumorinhibitionrateof32.87%，57.08%，and68.35%，respectively;thelow`-，middle`-，andhigh`-dosefruitbodypolysaccharidesfromphellinusigniariushadatumorinhibitionrateof44.14%，52.81%，and73.69%，respectively;thelow`-，middle`-，andhigh`-dosephellinusigniariuspolysaccharidesextractedfromfermentationbrothhadatumorinhibitionrateof49.16%，50.63%，and51.81%，respectively.conclusionallthreephellinusigniariusextractshaveastrongantitumoractivity，amongwhichfruitbodypolysaccharidesfromphellinusigniariushavethebestantitumoreffectandcansignificantlyincreaseth