穴位强化埋线疗法对STC患者直肠组织中ICC和神经元细胞的调节(1).pdfVIP

穴位强化埋线疗法对STC患者直肠组织中 ICC和神经元细胞的调节 作者：谢振年安晓静杨斌李东冰王芳丽苗春红冷涛贾小强赵卫兵曹威巍权隆芳原小千崔春晖 来源：《世界中医药》2020年第19期 摘要目的：探究穴位强化埋线疗法对慢传输型便秘（STC）直肠组织中ICC 细胞和神经元 的调节。方法：选择STC 患者41 例，非便秘患者41 例，分别设置为观察组和对照组。观察 组给予穴位强化埋线疗法，对照组未给予便秘相关治疗。比较观察组治疗前后直肠组织中ICC 细胞形态、相关蛋白干细胞因子（STem Cell FacTor，SCF）和c-kit mRNA 的表达水平和神经 元凋亡情况。结果：观察组患者经穴位强化埋线治疗后直肠组织中ICC 细胞的数量较治疗前增 加，且形态趋于正常。与对照组比较，SCF 和c-kit mRNA 在治疗前直肠组织中的表达明显降 低（P0.05 ，P0.01），而在治疗后直肠组织中的表达要高于治疗前（P0.01）。治疗前，观 察组患者直肠组织内神经元凋亡数量多于对照组（P0.01）。治疗后患者直肠组织内凋亡细胞 明显减少，与对照组比较差异有统计学意义（P0.05）。结论：穴位强化埋线可改善STC 患 者直肠组织中ICC 细胞的损伤，并减少神经元细胞的凋亡发生，从而改善便秘症状。 关键词穴位强化埋线;慢传输型便秘;Cajal 间质细胞;干细胞因子 Academy of Traditional Chinese Medicine， Beijing 100091 ， China; 3 Department of General Surgery， Aerospace Center Hospital ， Beijing 100083 ， China; 4 Beijing Anorectal Hospital， Beijing ， 102401 ， China; 5 Department of Anorectal Surgery， Pinggu District Hospital of Traditional Chinese Medicine， Beijing 101200 ， China） AbstractObjective ：To explore the regulation of acupoint intensive catgut embedding therapy on ICC cells and neurons in rectal tissue of slow transit constipation （STC）. Methods ：A total of 41 STC patients and 41 non-constipation patients were selected as the observation group and control group ， respectively. The patients in treatment group were given acupoint intensive catgut embedding therapy ， while the control group did not receive any constipation related treatment. The morphological of ICC and the expression of SCF and c-kit on RNA were compared before and after treatment in the treatment group. Results ：The number of ICC in the rectum tissue increased with the normal morphology after treatment. Compared with the control group， both SCF mRNA and c-kit mRNA in the rectum were significantly lower without treatment （P0.05 ， P0.01）. The expression in rectal tissue after treatment was higher than before treatment （P0.01）. Before treatment ， the number of neuronal apoptosis in the rectal tissue of the observation g