高通量酶抑制法快速测定蔬菜中有机磷和氨基甲酸酯类农药残留.pdfVIP

第14卷第3期 食品安全质量检测学报 Vol.14No.3 2023年2月 Journal of Food Safety and Quality Feb.,2023 高通量酶抑制法快速测定蔬菜中有机磷和 氨基甲酸酯类农药残留 王丽芳，王 雯，张 瑜，周钦方，王莲芳，王斌* （杭州市余杭区食品药品监测中心，杭州311199) 去从酶抑制法检测原 为，建立一种室温下高通量酶抑制法快速测定蔬菜中农药残留的方法。方法 摘要：目的 理出发，以阳性蔬菜样品为研究对象，通过对提取剂、提取时间及反应体系的选择和优化，确立酶反应体系。 采用该方法检测市售蔬菜样品，对筛选出阳性样品进行定量检测复核，验证方法有效性。结果 蔬菜样品用乙 作为提取液，采用超声提取，上清液氮吹后加人磷酸盐缓冲溶液(pH8.0)作为最终样液，农药提取效率最高， 且不会对酶抑制法反应体系造成影响。采用优化后的高通量农药速测方法，以室温(25±5)℃下酶抑制率≥40% 作为阳性判定标准，对筛选出的734个阳性样品进行仪器定量分析复核，检出符合率达到92.1%。结论 轮该方 法有效提升了农药提取效率和检测效率，可有效用于蔬菜中有机磷和氨基甲酸酯类农药残留的快速检测 关键词：高通量；酶抑制法；快速测定；农药残留 Rapid determination of organophosphorus and carbamate pesticide residues in vegetables by high-throughput enzyme inhibition method WANG Li-Fang, WANG Wen, ZHANG Yu, ZHOU Qin-Fang, WANG Lian-Fang, WANG Bin (Hangzhou Yuhang Food and Drug Monitoring Testing Center, Hangzhou 311199, China) ABSTRACT: ObjectiveTo establish a high-throughput enzyme inhibition method for the rapid determination of pesticide residues in vegetables at room temperature. MethodsBased on the principle of enzyme inhibition method, the enzyme reaction system was established through the selection and optimization of extractant, extraction time and reaction system with positive vegetable samples as the research object. This method was used to detect commercially available vegetable samples, and the positive samples were tested quantitatively to verify the effectiveness of the method. ResultsWithacetonitrile as the extraction solution, vegetabl