产生物碱暗紫贝母内生真菌A1次生代谢产物的研究.docVIP

  产生物碱暗紫贝母内生真菌 A1 次生代谢产 物的研究# 陈鹊，潘峰，吴卫** 5 10 15 20 25 30 35 40 （四川农业大学农学院，四川 成都，611130） 摘要：为研究产生物碱暗紫贝母内生真菌的次生代谢产物，本文以一株从暗紫贝母中分离得到的产生物碱 内生真菌 A1（三线镰刀菌）为材料，应用柱色谱技术，对其次生代谢产物进行了分离纯化，并以 5 种病原 菌（金黄色葡萄球菌，大肠杆菌，白色念珠菌，铜绿假单胞菌，粪肠球菌）为指示菌，采用琼脂孔法对其 纯化化合物的抑菌活性进行了研究。本试验共分离得到 5 种化合物，通过 1H-NMR 和 13C-NMR 谱数据及 MS 特征峰，鉴定出其中三种化合物，均为甾醇类化合物。抑菌活性结果显示，该 5 种化合物对 5 株指示 菌均无抑制作用。本文是首次报道从三线镰刀菌中获得甾醇类化合物，甾醇类化合物具有广泛的生理活性， 尽管本试验所得 5 种化合物并未显示出抑菌活性，但其它活性有待进一步探究。 关键词：真菌学；内生真菌；暗紫贝母；次生代谢；甾醇类化合物；抑菌活性 Study on the metabolites produced by an alkaloid-producing endophytic fungus strain A1 from Fritillaria unibracteata CHEN Que, PAN Feng, WU Wei (Agronomy College, Sichuan Agricultural University, Sichuan Chengdu 611130) Abstract: This paper aims to investigate the metabolites produced by alkaloid-producing endophytic fungi from Fritillaria unibracteata. Secondary metabolites were isolated and purified by silica gel column chromatography from the fermentation products of alkaloid-producing endophytic fungus strain A1 (Fusarium tricinctum) from F. unibracteata, And antimicrobial activities of these compounds on bacteria (Staphyloccocus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Enterococcus faecalis) have been tested in agar-well diffusion methods. Five compounds had been isolated and purified, and three of them were respectively confirmed as 5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol, 24(R)-methylcholesta-5-en-3β-ol(campesterol), 24(R)-methylcholesta-5,22-en-3β-ol (brassicasterol) according to mass spectrometer (MS) and nuclear magnetic resonance (NMR) spectroscopy. But the structures of the rest were still undetermined due to insufficient reference information. In addition, the results of antimicrobial activities of these compounds revealed they did not play a positive role in inhibiting the growth of five strain bacteria. It is firstly reported that the sterols were isolated from the metabolites of F. tricinctum. Besides, it is worth investigating further these s