可水解型羧酸甜菜碱酯表面的构筑及性能.docVIP

  可水解型羧酸甜菜碱酯表面的构筑及性能# 史素青，张琴，赵洋，宫永宽* （合成与天然功能分子化学教育部重点实验室,西北大学化学与材料科学学院,西安 710069） 5 10 15 20 摘要：采用(N,N-二甲基-3-氨丙基)三甲氧基硅烷（DATS）对羟基化表面进行硅烷化处理引 入叔胺功能基团，然后与溴乙酸乙酯进行季铵化反应构筑羧酸甜菜碱酯季铵阳离子表面 （Glass- N+(CH3)2(CH2COOEt)），以接触角为考察指标，研究了溴乙酸乙酯浓度、反应温度 和反应温度对表面季铵化反应的影响，结果发现，当溴乙酸乙酯质量分数为 20%、反应温 度为 7 0、反应时间为 24h 时，所得羧酸甜菜碱酯表面的亲水性最佳，前进角和后退角分 别为 35°和 17°。当 Glass- N+(CH3)2(CH2COOEt) 表面在不同浓度氨水中水解后表面亲水 性基本不变，但蛋白质吸附量和血小板黏附量却随着氨水浓度的增大而逐级降低；与 Glass- N+(CH3)2(CH2COOEt)，当氨水浓度为 0.4mol/L 时，水解后表面纤维蛋白原（Fg）和牛血清 蛋白（BSA）的吸附量分别降低了 92.4%和 90.3%，血小板基本不发生黏附。 关键词：羧酸甜菜碱酯表面; 水解；季铵化; 表面性能 中图分类号：O631 Preparation and properties of hydrolysable carboxybetaine ester modified surface SHI Suqing, ZHANG Qin, ZHAO Yang, GONG Yongkuan (Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, College of Chemistry Materials Science, Northwest University, Xian 710069, China) Abstract: A hydrolysable cationic carboxybetaine ester modified surface (Glass- N+(CH3)2(CH2COOEt)) is fabricated by the quaternization of surface immobilized tertiary amine 25 30 35 40 (by silinization) and ethyl bromoacetate. The influences of concentration of ethyl bromoacetate, reaction temperature and time on the quaternization reaction are evaluated by the changes of water contact angles. The results show that the optimal hydrophilicity of Glass- N+(CH3)2(CH2COOEt can be achieved under the following conditions: 20% mass fraction of ethyl bromoacetate, 70℃ of temperature and 24h of reaction time. Then the obtained Glass- N+(CH3)2(CH2COOEt) is hydrolyzed in different concentrations of ammonium water. Although the hydrophilicity of the hydrolyzed surfaces almost does not change by increasing the concentrations of ammonium water from 0.1 mol/L to 0.4 mol/L, the amounts of absorbed proteins are gradually reduced. Particularly, when the concentration of ammonium water is 0.4 mol/L, the adsorption of Fg and BSA is reduced by 93% and 90%, and the platelet adhesion does not occur, which indicates that the cont