雷帕霉素靶标TOR 结合蛋白Raptor2 参与调控植物叶绿体形成过程.docVIP

  雷帕霉素靶标 TOR 结合蛋白 Raptor2 参 与调控植物叶绿体形成过程# 于永华，孙林啸，闵启明，洪月云** 5 10 15 （华中农业大学作物遗传改良国家重点实验室，武汉 430070） 摘要：雷帕霉素靶标蛋白 TOR（Target of rapamycin）系 Ser/Thr 激酶，动物 mTOR 广泛参 与各种不同的生物过程，是调控细胞分裂和生长的主导者。 Raptor（Regulatory - Associated Protein of TOR）是 TOR 复合体 TORC1 的重要成员，动物 Raptor 通过募集 TOR 下游蛋白， 促进 TOR 对其下游蛋白的磷酸化和活化，在细胞增殖和生长过程中至关重要。 拟南芥 AtRaptor1 基因的缺失突变使胚早期致死， 但植物 Raptor 在胚后植株生长发育过程中的作 用尚未清楚。本研究通过基因克隆、蛋白表达、缺失突变和 RNAi 等方面分析水稻 Raptor2 在植物生长发育中的作用。结果表明水稻 Raptor2 的突变导致植株叶绿体发育受阻，raptor2 突变体叶片出现完全或部分白化，其白化叶片叶绿素 a 和 b 含量分别比野生型减少了 45% 和 44%，导致突变体植株生长明显滞后，表明 Raptor2 对植物叶绿体形成和发育具有正调控 作用。此外，本研究还从叶片组织 mRNA 反转录扩增了 Raptor2 全长 cDNA, 并在大肠杆菌 细胞中表达了 Raptor2 蛋白，为后续研究 TOR 信号的分子调控机制奠定了基础。 关键词：植物生长；水稻 Raptor2； 雷帕霉素靶标蛋白 TOR； 叶绿体形成 中图分类号：Q756 20 Raptor2, the Regulatory Associated Protein of TOR, is Involved in Chloroplast Biogenesis in Rice YU Yonghua, SUN Linxiao, MIN Qiming, HONG Yueyun (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 25 30 35 40 Wuhan 430070) Abstract: Target of Rapamycin (TOR) is a conserved Ser/Thr kinase involved a various biological processes, and is a master regulator in proliferation and growth. Regulatory - Associated Protein of TOR （Raptor） is a component of TOR complex 1 and also plays an important role in growth regulation. Arabidopsis contains two Raptor genes. It was showed that loss of Raptor1 resulted in embryonic lethality in Arabidopsis. However, it is not clear the involvement of Raptor in post-embryonic plant growth. To characterize the roles of Raptor in plant growth and development, this study was undertaken to explore the molecular regulation of Raptor2 via genetic manipulation in rice. The result showed that the loss of Raptor2 conferred leaves became pale or pale-green strips. The contents of chlorophyll a and b in raptor2 mutant plants were reduced by 45% and 44%, respectively. As a result, raptor2 mutant plants grew much slower than wild type. Further observation showed that chloroplast is disrupted in raptor2 mut