生长素对harpinXoo蛋白激发HR和ROS的影响.docVIP

  生长素对 harpinXoo 蛋白激发 HR 和 ROS 的影响# 宋洁，高青，宋风雅，谢琳琳，郑服丛，缪卫国** 5 10 15 20 25 30 （海南省热带生物资源可持续利用重点实验室/海南大学环境与植物保护学院，海南海口 570228） 摘要：Harpin 蛋白能激发植物产生过敏反应( hypersensitive response, HR )，然而植物产生过 敏反应的机制尚不清楚。本文在烟草叶片上测定了，在未直接涂抹生长素吲哚乙酸 ( Indole-3-aceticacid, IAA )的部分叶片上，IAA 对 harpinXoo 蛋白引起的 HR 的影响。烟草表 型试验表明 10-4 mol/L IAA 能够逆转未直接涂抹 IAA 处 harpinXoo 激发的 HR，而 500 μ M 生长素抑制剂三碘苯甲酸(2,3,5-trllodobenzolc acid, TIBA)能够抑制 10-4 mol/L IAA 的作用。 与烟草 HR 表型试验结果一致，对烟草叶组织内的氧爆发现象进行了测定，IAA 同样减少了 活性氧(ROS)的积累。结论为，IAA 可以逆转 harpinXoo 蛋白引起的 HR，也可以调控氧爆 发。 关键词：HarpinXoo；IAA；过敏反应；活性氧；逆转 HR 中图分类号：Q945.8 IAA Affects Harpin-induced Hypersensitive Response and ROS Song Jie, Gao Qing, Song Fengya, Xie Linlin, Zheng Fucong, Miao Weiguo (Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection，Hainan University，Haikou 570228，China) Abstract: Harpin proteins can stimulate hypersensitive response (HR) on plants, but now, how plants regulate the mechanism of hypersensitive response is not clear. This paper researches whether indole-3-aceticacid (IAA) can repress the HR stimulated by harpin protein on tobacco leaves without coated with IAA directly. Plant assays showed that 10-4 mol/L IAA can reverse the HR stimulated by harpinXoo protein on tobacco leaves without coated with IAA directly, and the effect can be inhibited by applying 500 μM 2, 3, 5-trllodobenzolc acid (TIBA) at the same time. Then we determined the condition of oxidative burst of tobacco leaf tissues. The results were in accordance with the results of plant assays that IAA could reduce the accumulation of ROS. Auxin can reverse the hypersensitive response stimulated by harpinXoo protein on tobacco leaves without coated with IAA directly. Key words: HarpinXoo; IAA; hypersensitive response; reactive oxygen species; reverse HR 35 0 引言 Harpin由革兰氏阴性植物病原细菌产生，并通过III型泌出通道分泌，是一种能够引起非 寄主植物产生过敏反应(hypersensitive response, HR ) [1]的蛋白质激发子。大部分harpin注射烟 草叶片或处理烟草悬浮细胞，都会诱发植物过敏性细胞死亡[2-3]，还可以检测到HR标志基因