树枝状聚合物涂层对釉质表面晶体结构再生的作用.docVIP

  树枝状聚合物涂层对釉质表面晶体结构再 生的作用#  1,2  1,2**  5 10 15 20 25 30 35 40 45  （1. 口腔疾病研究国家重点实验室，四川成都，610041； 2. 四川大学华西口腔医院牙体牙髓科，四川成都，610041） 摘要：目的：本实验的目的为在接近临床使用的反应环境中，研究自制的 PAMAM 作为有机模板诱导牙釉质 表面晶体结构原位再生的作用，探索其影响因素和作用机制，为后续 PAMAM 诱导牙釉质原位仿生修复奠定 基础。方法：通过涂层处理的方法在酸蚀后的牙釉质表面形成 PAMAM 模版层，再将处理后的样本至于矿化 溶液中静置反应 20 小时，利用外吸收光谱（FTIR）、激光共聚焦显微镜（CLSM）、X 射线衍射（XRD）、 扫描电镜（SEM）观察在 PAMAM 模版剂作用下新生晶体的成分、尺寸和形态。结果：FITR 结果显示新生晶 体在 1650 cm-1、2926 cm-1、2854 cm-1 左右有明显的 PAMAM 特征峰出现； XRD 结果显示：涂层法中釉质 表面新成的 HAP 晶体平均长度为 38.27nm，宽度为 21.67nm，长宽比为 1.76；SEM 结果显示：新生晶体均为 短棒状，生长方向与原位釉质晶体生长方向一致。结论：PAMAM 能促进牙釉质样晶体形成，并且可以调控 晶体沿原位牙釉质晶体方向生长，可作为牙硬组织仿生矿化的模版剂。 关键词： 口腔临床医学；树枝状聚合物；PAMAMA；羟磷灰石晶体；仿生矿化。 中图分类号：R781.1 STUDY OF CRYSTAL GROWTH IN THE PRESENT OF PAMAM ON THE ENAMEL SURFACE CHEN Liang1,2, LI Jiyao1,2 (1. Department of Endodontics and Operative Dentistry, West China School of Stomatology, Sichuan University, 610041; 2. State Key Laboratory of Oral Diseases,Sichuan University, 610041) Abstract: Objective: To repair the demineralized enamel by biomimetic method, and the effect of Poly (amido amine) (PAMAM) dendrimers on the crystallization of hydroxyapatite on etched enamel surface is investigated. Design: PAMAM dendrimers were synthesized step by step following the classical method and modified with the carboxylic acid groups (COOH) on the surface. Demineralized human enamel samples were immersed in 10,000 ppm PAMAM-COOH solution for 30 min and then in calcium phosphorous solution with or without fluoride under near-clinical conditions for 20 h. Other samples without PAMAM-COOH were immersed in calcium phosphorous solution as the control group. After the immersion, the micro structure, morphology and composition of the regrown crystals on the longitudinal and transversal enamel surfaces were investigated by SEM, XRD and FTIR, and the results were compared with etched enamel and intact enamel. Results: With the PAMAM-COOH templates, well-arranged rod-like crystals were formed and they were parallel to the long axis