微小RNA 29对人肺腺癌侧群细胞增殖和克隆形成的影响.docVIP

  微小 RNA 29 对人肺腺癌侧群细胞增殖和克 隆形成的影响# 孙婧，束永前** 5 10 15 20 （南京医科大学第一附属医院肿瘤科，南京，江苏，210029） 摘要：目的：探讨微小 RNA 29 是否影响人肺腺癌侧群细胞的增殖和克隆。方法：利用 Hoechst33342 染料法，通过流式细胞仪紫外激光分选功能分选出人肺腺癌 A549 细胞中的 SP 细胞；使用 Liptamine 2000 系统将 hsR-miR-29、miR 29 siRNA 转入 SP 细胞，real-time PCR 检测 miR 29 在 SP 细胞内的表达； MTS 法检测转染后细胞吸光值的变化；细胞克隆实验观 察转染后 SP 细胞的增殖差异。结果：转染 hsR-miR-29、miR 29 siRNA 后可上调或下调 miR 29 的表达；转染 hsR-miR-29 后 SP 细胞吸光值和细胞克隆形成数较对照组明显减少，而转 染 miR 29 siRNA 组吸光值和细胞克隆数显著增加。 结论：miR 29 可调控人肺癌侧群细胞 的增殖，下调其表达会促进其增殖，而上调其表达则抑制细胞增殖。 关键词：微小 RNA 29；肺癌；侧群细胞；增殖；克隆形成 中图分类号：请查阅《中国图书馆分类法》 microRNA 29 can affect the proliferation and colony formation of human lung adenocarcinoma side population cells. Sun Jing, Shu Yongqian (Department of Medical Oncology, The First Affiliated Hospital of Nanjing Medical University,Nanjing, Jiangsu Province, 210029) Abstract: Objective: To investigate whether miR 29 can regulate the proliferation and colony formation of human lung adenocarcinoma side population (SP) cells; Methods: SP cells were 25 30 isolated using flow cytometry and Hoechst 33342 dye efflux assay from human lung adenocarcinoma A549 cells. hsR-miR 29, miR 29 siRNA were transfected into SP cells using Liptamine 2000 systems and the expression of miR 29 was measured by real-time PCR. MTS test was used to observe the proliferation differences between cells after transfection. Colony formation was applied to confirm the differences between the groups. Results: The expression of miR 29 can be successfully upregulated or downregulated by transfection; the absorbance value and colony numbers of SP cells with hsR-miR-29 transfection are much less than control, while the miR 29 siRNA group are much more. Conclusion: miR 31 regulates the sensitivity of human lung cancer side population (SP) cells to cisplatin through RhoBTB1. It should be a new mechanism and target for the cancer research of drug resistance. 35 Key words: microRNA 29; lung cancer; side population cells; proliferation; colony formation  0 引言 肺癌是严