小干扰RNA抑制肝癌细胞 胰岛素样生长因子II基因表达的初步研究.pdfVIP

RNA II IGF-II siRNASMMC-7721 IGF-II II mRNA siRNA IGF-II mRNA 3 siRNA SMMC-7721FAM siRNA siRNA4 siRNA13 SMMC-7721 siRNA4 siRNA MTT siRNA RT-PCR siRNA IGF-II mRNA FAM siRNA 6580 siRNA12 3 siRNA4 MTT P0.01siRNA3 siRNA3 :10nM20nM 40nM 80nM siRNA3 RT-PCR siRNA l0nM mRNA 40nM 86.34 40nM siRNA13 siRNA3 siRNA siRNA35- GUUCUUCCAAUAUG ACACC -35- GGUGUCAUA UUGGAAGA AC -3 IGF-IImRNA siRNA SMMC-7721siRNA siRNA IGF-IImRNA IGF-II RNA RNA II 1 RNA II ABSTRACT Objective: To design and construct siRNAs targeting human IGF-IIand observe their influences on the proliferation of cells and inhibition of the expression of IGF-II mRNA in SMMC-7721 cells. To specify the siRNAs sequence and its concentration of the effective gene therapy through siRNAs targeting IGF-II in hapatocellular carcinoma. This paper tries to provide scientific evidence for gene therapy on hapatocellular carcinoma. Methods: Three siRNAs targeting human IGF-II marked with FAM, and one siRNA(siRNA4) without any targeted gene (used for a negetive control) were designed and chemically synthesized. Then the synthetic siRNAs were transfected into SMMC-7721 cells by lipofectamin. The comparison would be made between the siNRA1~3 group and the siRNA4 group, the untransfected group and the lipofectamin group. The efficiency of