菌株Ⅰ13产碱性蛋白酶的酶学性质研究（摘要）.pdfVIP

AgriculturalScience ＆Technology 2011 ， 12(2)：163—164．170 Copyright~)2011，InformationI4ns．t,itu-te-ofHAAs Allrightsreserved ． AgriculturalBasicScienceandMethod PropertiesofAlkalineProteaseProducedbyStrainI13 GU Yan,li，LIU Sai-nan，ZHANG Hui，LlXian-zhen SchoolofBiOengineer¨1g，DalianPolytechnicUniversity，Dalian 116034 Abstract objectivelThisstudyaimedtoinvestigatingpropertiesofalkalineproteaseproducedbystrainl13 M『ethod]Crudeenzymeofal- kaline proteasewasobtainedfr0m alkalineproteaseproduced bystrainl13．whileeffectsoftemperatureandpH valueonenzymeactivitywere alsoinvestigatedinthisstudy．IResultITheoptimaltemperatureofalkalineproteaseproducedbystrainl13was40 ℃，whileenzymeactivitv maintainsahigherleveIfrom3Ot06O℃ andover40％ ofthelargestenzymeactivitystilImaintainedwithintherangefrom2OtO30℃．Theopti． maIpH valuewas10．5．andover9O％ ofthelargestenzymeactivitystilImaintainedwithintherangefr0m 8．OtO 11．0．which hadbroaderpH valuespectrum ．fConclusionfThisalkalineproteasehashugepotentiaftobedevelopedintowashing—powderadditive． Keywords AIkalineprotease：Screening；Enzymaticcharacterization ASthecatalyst．alkalineprotease hasbeenwidely used 20℃ with160r／min．afterthattheculturewithO =0．15 intan，silverrecovery，medicine，food processnig，forage， wasinoculated intofermentationmedium with5％ ofInocula- chemicalindustryandwastetreatment．Especially．itiswidely tionamountforshakecultureat20oC with160 r／minfor36h ． usedasnon—phosphidedetergentadditive … ．However．de— andthenthesupernatantwasusedasthecrudeenzyme． tergentdemandsthehigherenzymeactivityandstabilityofal— Enzymeactivity measurement Referringto protease test kal