鲤鱼白细胞介素-1β全长cDNA的克隆·鉴定及其差异表达分析（摘要）（英文）.pdfVIP

AgriculturalScience＆Technology，2011，12(3)：405—408，412 Copyright⑥2011，InformationInstituteofHAAS Allrightsrese~ed AnimalScience Cloning，Identification and DifferentialExpression AnalysisofFull--lengthcDNAofCarpInterleukin-113 HEJiang-shuai’ LU Qiang ，LIWei，ZHAO Xiao’ FENG Xiang—ru’ CHEN Yi-long’ ， ， ， 1．MinistryofEducationKeyLaboratoryforZoonosis，JUinUniversity，Changchun130062；2．HeilongjiangVocationalCollegeofBiology ScienceandTechnology，Harbin150025 Abstract fobjective1Theresearchaimedtocarry outthecloning，identificationanddifferentialexpressionanalysisofcarpinterleukin-1B (IL一131)cDNA．[Method]ByusingDD-RTPCRmethod，thedifferentialexpressioncDNAfracImentsweregained．ThecDNAlibraryofcarppe- ripheralbloodleucocyteswhichwasstimulatedbythemitogenwasscreened．andthefulIlengthcDNAofcarplL-113wascloned．Moreover．the sequenceanalysisanddifferentialexpressionanalysiswerecarriedout．IResult1ThepositiveclonewhichhadawholeORFthatencoded276a． minoacidswasobtained．TheclusteranalysisshowedthattheaminoacidsequenceofcarplL-131andJapanesecarpcloselygatheredasa branch．andthe homoeologyofaminoacidsequencereached95％ ．Theclusteringorderwasthecarassius，zebrafish，pig．cable，horse．hu— manandmouseinturn．ThedifferentiaJexpressionanalysisshowedthattheexpressionoflL-131intheleucocytassignificantlyjncreasedinthe priorperiod (4h)afferthemitogenstimulated．Butasthetimewentbv(12and24h)．itdidn’tincreaseinthesameperiod．Thetotaltrendof expressionamountpresentedthepeaktype．IConclusionlTheresearchIaidthefoundationforfurtherstudyingtheexpressionmanner．function characteristic．regulationmechanism oflL一131门『vivoandjtsactionmechanismsintheinflammatoryreaction．emergencyreactionandimmunere- sponse． Keywords Carp：Interleukin-131；cDNAcloning：ldentification；DifferentiaIexpressionanalysis IL．113wasfirstlyfoundwhenstudyingtheimmunesystem