利用体细胞核移植技术生产表达红色荧光蛋白的猪转基因克隆胚胎（摘要）.pdfVIP

AgriculturalScience＆Technology，2011，12(2)：287—290，307 Copyright⑥2011，InformationInstituteofHAAS．Allrightsresewed AnimaIScience ProductionofTransgenicPig CloneEmbryo ExpressedtheRed FluorescentProteinby UsingtheSomaticCellNucleus TranSplantationTechnology KANG Jin—dan’ YINXi—jun’，ZHAOMing-hui，LIANGShuang’，LIUXi’，LIWen-xue，CUlCheng—zhe。 ， 1．InstituteofAnimalEmbryoEngineeringinYanbianUniversity，Yanji133002；2．AgriculturalCollegeofYanbianUniversity，Yanji133002； 3．YanbianResearchAcademyofAgriculturalSciences，Longjing133400 Abstract J0biectivejTheresearchaimedtolaythefoundationforproducingthetransgenicclonepig．MethodlThepigfetusfibroblastwith theredfluorescentprotein (RFP)genethatwastransfectedbytheretroviruswasasthedonorofnucleustransplantation．Byusingthesomatic cellcloningtechnology，thedevelopmentsituation ／nvitroofcloneembryowithRFPwasstudied．1Resuit]ThefusionrateofRFPtransgeniccel1 was83．87％ whichhadnosignificantdifferencewith80．56％ ofnon·transgeniccelI(P0．05)．TheblastularateinvitroofRFPtransgenicso- maticcelIreconstructedembryowas8．67％ whichhadnosignificantdifferencewith6．56％ ofnon·transgeniccellfP0．O51．Aftertherecon— structedembryoofRFPtransgenicsomaticcelfwastransplantedintofifteenreceptors．therewasnoconception individua1．fConclusion]The transgeniccellwiththeredfluorescentproteinasthedonorcouldsuccessfullyclonethetransgenicembryoandobtainthetransgenicblastula． Keywords Transgene；Redfluorescentprotein；Nucleustransplantation；Pig Thepig snotonlythemostimportantmeatanimaIbutal— ment．celIcomponentandhad the higherSNR．Moreover． so s the importantdisease modeIanimaIof human．The the intracellularfluorescence conversion efficiencywashigh． physiologicalcharacteristicofpig isverysimilartothatofhu· er，whichwas easytodetect．Atpresent，RFP wasapplied man ，such as in the toxicology，dermatology，diabetes， into the transgene， protein an